IGEM:Virginia 2012/Protocols/Phage Replication Rate Assay - Revision history

Joseph J. Muldoon: /* Overview */

2012-05-30T03:32:15Z

Overview

←Older revision		Revision as of 03:32, 30 May 2012
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	==Overview==		==Overview==

-	Below is the procedure for measuring the rate of replication of a phage inside a certain organism. The bacteriophage and the organism are cultured together, and ~~sample~~ are taken and titered at 0, 3, and 6 hours.	+	Below is the procedure for measuring the rate of replication of a phage inside a certain organism. The bacteriophage and the organism are cultured together, and samples are taken and titered at 0, 3, and 6 hours.

	==Materials==		==Materials==

Jacqueline K. Grimm at 20:50, 29 May 2012

2012-05-29T20:50:47Z

←Older revision		Revision as of 20:50, 29 May 2012
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	# Take 1 mL from the bacterial culture and add it to ~20 mL of liquid LB medium.		# Take 1 mL from the bacterial culture and add it to ~20 mL of liquid LB medium.
	# Add phage suspension to the bacterial culture.		# Add phage suspension to the bacterial culture.
-	# Take a 500 μl sample and put it in the ~~freezer~~.	+	# Take a 500 μl sample and put it in the 4 degree fridge.
	# Put the bacteria/phage mixture in the incubator.		# Put the bacteria/phage mixture in the incubator.
-	# Take 500 μl samples and put them in the ~~freezer~~ after 3 hours and 6 hours.	+	# Take 500 μl samples and put them in the 4 degree fridge after 3 hours and 6 hours.
	# For each of the three samples, [http://openwetware.org/wiki/IGEM:Virginia_2012/Protocols/Phage_Isolation isolate the phage].		# For each of the three samples, [http://openwetware.org/wiki/IGEM:Virginia_2012/Protocols/Phage_Isolation isolate the phage].
	# For each of the three samples, perform a [http://openwetware.org/wiki/IGEM:Virginia_2012/Protocols/Plaque_Assay plaque assay] to determine the phage titer.		# For each of the three samples, perform a [http://openwetware.org/wiki/IGEM:Virginia_2012/Protocols/Plaque_Assay plaque assay] to determine the phage titer.

Jacqueline K. Grimm: New page: ==Overview== Below is the procedure for measuring the rate of replication of a phage inside a certain organism. The bacteriophage and the organism are cultured together, and sample are ta...

2012-05-29T17:18:04Z

New page: ==Overview== Below is the procedure for measuring the rate of replication of a phage inside a certain organism. The bacteriophage and the organism are cultured together, and sample are ta...

New page

==Overview==

Below is the procedure for measuring the rate of replication of a phage inside a certain organism. The bacteriophage and the organism are cultured together, and sample are taken and titered at 0, 3, and 6 hours.

==Materials==

*Liquid LB medium
*Bacterial culture
*Phage suspension

==Procedure==

# Take 1 mL from the bacterial culture and add it to ~20 mL of liquid LB medium.
# Add phage suspension to the bacterial culture.
# Take a 500 μl sample and put it in the freezer.
# Put the bacteria/phage mixture in the incubator.
# Take 500 μl samples and put them in the freezer after 3 hours and 6 hours.
# For each of the three samples, [http://openwetware.org/wiki/IGEM:Virginia_2012/Protocols/Phage_Isolation isolate the phage].
# For each of the three samples, perform a [http://openwetware.org/wiki/IGEM:Virginia_2012/Protocols/Plaque_Assay plaque assay] to determine the phage titer.

==Notes==
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!



==References==


==Contact==
*Who has experience with this protocol?

or instead, [[Talk:{{PAGENAME}}|discuss this protocol]].


[[Category:Protocol]]