IGEM:Virginia 2012/Protocols/Preparing a Pertussis Culture
Below is the procedure for preparing a Bordetella pertussis culture from frozen stock. First, the bacteria will be grown on a plate for ~3 days. Then it will be grown in liquid culture for a day, and then diluted so that the bacteria will be in log phase a day later. This procedure should be done in a hood to prevent aerosolization.
- Pertussis frozen stock
- Sheep's blood agar plates
- Sterilized sticks
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
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