IGEM:Virginia 2012/Protocols/SM Buffer Preparation
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< IGEM:Virginia 2012 | Protocols(Difference between revisions)
(New page: ==Materials== *2.9 g NaCl *0.49 g MgSO4 *25 mL 1 M Tris*Cl pH 7.5 *BTV 500 mL dH2O ==Procedure== ==Notes== Please feel free to post comments, questions, or improvements to this protocol...) |
Current revision (09:40, 26 July 2012) (view source) |
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*2.9 g NaCl | *2.9 g NaCl | ||
*0.49 g MgSO4 | *0.49 g MgSO4 | ||
| - | *25 mL 1 M Tris*Cl pH 7.5 | + | *25 mL 1 M Tris*Cl at pH 7.5 |
| - | * | + | *less than 500 mL of distilled H2O |
==Procedure== | ==Procedure== | ||
| + | * Add the NaCl and MgSO4 to a 1 L bottle. | ||
| + | * Add in 25 mL of 7.5 pH Tris-Cl | ||
| + | * Bring this to a volume of 500 mL by adding distilled water. | ||
| + | * Autoclave (large liquid). | ||
==Notes== | ==Notes== | ||
Current revision
Contents |
Materials
- 2.9 g NaCl
- 0.49 g MgSO4
- 25 mL 1 M Tris*Cl at pH 7.5
- less than 500 mL of distilled H2O
Procedure
- Add the NaCl and MgSO4 to a 1 L bottle.
- Add in 25 mL of 7.5 pH Tris-Cl
- Bring this to a volume of 500 mL by adding distilled water.
- Autoclave (large liquid).
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
References
Contact
- Who has experience with this protocol?
or instead, discuss this protocol.
