IGEM:Virginia 2012/Protocols/SM Buffer Preparation

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(Procedure)
Line 10: Line 10:
*  Add in 25 mL of 7.5 pH Tris-Cl
*  Add in 25 mL of 7.5 pH Tris-Cl
* Bring this to a volume of 500 mL by adding distilled water.
* Bring this to a volume of 500 mL by adding distilled water.
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* Be happy.
+
* Autoclave (large liquid).
==Notes==
==Notes==

Revision as of 13:09, 30 May 2012

Contents

Materials

  • 2.9 g NaCl
  • 0.49 g MgSO4
  • 25 mL 1 M Tris*Cl at pH 7.5
  • less than 500 mL of distilled H2O

Procedure

  • Add 2.9g of NaCl to a 1 L bottle.
  • Add in 25 mL of 7.5 pH Tris-Cl
  • Bring this to a volume of 500 mL by adding distilled water.
  • Autoclave (large liquid).

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!


References

Contact

  • Who has experience with this protocol?

or instead, discuss this protocol.

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