IGEM:Virginia 2012/Protocols/SM Buffer Preparation: Difference between revisions
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* Add in 25 mL of 7.5 pH Tris-Cl | * Add in 25 mL of 7.5 pH Tris-Cl | ||
* Bring this to a volume of 500 mL by adding distilled water. | * Bring this to a volume of 500 mL by adding distilled water. | ||
* | * Autoclave (large liquid). | ||
==Notes== | ==Notes== | ||
Revision as of 11:09, 30 May 2012
Materials
- 2.9 g NaCl
- 0.49 g MgSO4
- 25 mL 1 M Tris*Cl at pH 7.5
- less than 500 mL of distilled H2O
Procedure
- Add 2.9g of NaCl to a 1 L bottle.
- Add in 25 mL of 7.5 pH Tris-Cl
- Bring this to a volume of 500 mL by adding distilled water.
- Autoclave (large liquid).
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
References
Contact
- Who has experience with this protocol?
or instead, discuss this protocol.
