IGEM:Virginia 2012/Protocols/SM Buffer Preparation

Materials

• 2.9 g NaCl
• 0.49 g MgSO4
• 25 mL 1 M Tris*Cl at pH 7.5
• less than 500 mL of distilled H2O

Procedure

• Add the NaCl and MgSO4 to a 1 L bottle.
• Add in 25 mL of 7.5 pH Tris-Cl
• Bring this to a volume of 500 mL by adding distilled water.
• Autoclave (large liquid).

Notes

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