IGEM:Virginia 2012/Protocols/SSM Media Preparation
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| KH<sub>2</sub>PO<sub>4</sub> || 0.50g || 1.00g || 2.00g || 2.50g | | KH<sub>2</sub>PO<sub>4</sub> || 0.50g || 1.00g || 2.00g || 2.50g | ||
|- | |- | ||
| - | | KCl || 0.20g || 0.40g || 0.80g || 1.00g | + | | KCl ''*200 mg/mL'' || 0.20g || 0.40g || 0.80g || 1.00g |
|- | |- | ||
| - | | MgCl<sub>2</sub> 6H<sub>2</sub>O || 100mg || 200mg || 400mg || 500mg | + | | MgCl<sub>2</sub> 6H<sub>2</sub>O ''*100mg/mL (0.5 mM)'' || 100mg || 200mg || 400mg || 500mg |
|- | |- | ||
| - | | CaCl<sub>2</sub> 2H<sub>2</sub>O || 20mg || 40mg || 80mg || 100mg | + | | CaCl<sub>2</sub> 2H<sub>2</sub>O ''*20 mg/mL'' || 20mg || 40mg || 80mg || 100mg |
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| + | |||
| + | *Make stock solutions and add this amount. | ||
==Procedure== | ==Procedure== | ||
Revision as of 14:27, 31 May 2012
Contents |
Materials
| BASAL MEDIUM to make | 1 Liter | 2 Liters | 4 Liters | 5 Liters |
| Glutamic acid, monosodium salt, 1H2O | 10.72g | 21.44g | 42.88g | 53.60g |
| Trisma Base | 1.525g | 3.05g | 6.10g | 7.63g |
| NaCl | 2.50g | 5.00g | 10.00g | 12.50g |
| KH2PO4 | 0.50g | 1.00g | 2.00g | 2.50g |
| KCl *200 mg/mL | 0.20g | 0.40g | 0.80g | 1.00g |
| MgCl2 6H2O *100mg/mL (0.5 mM) | 100mg | 200mg | 400mg | 500mg |
| CaCl2 2H2O *20 mg/mL | 20mg | 40mg | 80mg | 100mg |
- Make stock solutions and add this amount.
Procedure
- Add 2.9g of NaCl to a 1 L bottle.
- Add in 25 mL of 7.5 pH Tris-Cl
- Bring this to a volume of 500 mL by adding distilled water.
- Autoclave (large liquid).
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
References
Contact
- Who has experience with this protocol?
or instead, discuss this protocol.
