IGEM:Virginia 2012/Protocols/SSM Media Preparation

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Line 12: Line 12:
| KH<sub>2</sub>PO<sub>4</sub> || 0.50g || 1.00g || 2.00g || 2.50g
| KH<sub>2</sub>PO<sub>4</sub> || 0.50g || 1.00g || 2.00g || 2.50g
|-
|-
-
| KCl || 0.20g || 0.40g || 0.80g || 1.00g
+
| KCl ''*200 mg/mL'' || 0.20g || 0.40g || 0.80g || 1.00g
|-
|-
-
| MgCl<sub>2</sub> 6H<sub>2</sub>O || 100mg || 200mg || 400mg || 500mg
+
| MgCl<sub>2</sub> 6H<sub>2</sub>O   ''*100mg/mL (0.5 mM)'' || 100mg || 200mg || 400mg || 500mg
|-
|-
-
| CaCl<sub>2</sub>  2H<sub>2</sub>O || 20mg || 40mg || 80mg || 100mg
+
| CaCl<sub>2</sub>  2H<sub>2</sub>O   ''*20 mg/mL'' || 20mg || 40mg || 80mg || 100mg
|}
|}
 +
 +
*Make stock solutions and add this amount.
==Procedure==
==Procedure==

Revision as of 13:27, 31 May 2012

Contents

Materials

BASAL MEDIUM to make 1 Liter 2 Liters 4 Liters 5 Liters
Glutamic acid, monosodium salt, 1H2O 10.72g 21.44g 42.88g 53.60g
Trisma Base 1.525g 3.05g 6.10g 7.63g
NaCl 2.50g 5.00g 10.00g 12.50g
KH2PO4 0.50g 1.00g 2.00g 2.50g
KCl *200 mg/mL 0.20g 0.40g 0.80g 1.00g
MgCl2 6H2O *100mg/mL (0.5 mM) 100mg 200mg 400mg 500mg
CaCl2 2H2O *20 mg/mL 20mg 40mg 80mg 100mg
  • Make stock solutions and add this amount.

Procedure

  • Add 2.9g of NaCl to a 1 L bottle.
  • Add in 25 mL of 7.5 pH Tris-Cl
  • Bring this to a volume of 500 mL by adding distilled water.
  • Autoclave (large liquid).

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!


References

Contact

  • Who has experience with this protocol?

or instead, discuss this protocol.

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