IGEM:Virginia 2012/Protocols/SSM Media Preparation: Difference between revisions
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==Materials== | |||
{| border="0" cellpadding="0" | {| border="0" cellpadding="0" | ||
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| CaCl<sub>2</sub> 2H<sub>2</sub>O || 20mg || 40mg || 80mg || 100mg | | CaCl<sub>2</sub> 2H<sub>2</sub>O || 20mg || 40mg || 80mg || 100mg | ||
|} | |} | ||
==Procedure== | |||
* Add 2.9g of NaCl to a 1 L bottle. | |||
* Add in 25 mL of 7.5 pH Tris-Cl | |||
* Bring this to a volume of 500 mL by adding distilled water. | |||
* Autoclave (large liquid). | |||
==Notes== | |||
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input! | |||
<!-- Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.--> | |||
==References== | |||
<!-- If this protocol has papers or books associated with it, list those references here.--> | |||
==Contact== | |||
*Who has experience with this protocol? | |||
or instead, [[Talk:{{PAGENAME}}|discuss this protocol]]. | |||
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[[Category:Protocol]] | |||
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[[Category:Needs attention]] | |||
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[[Category:DNA]] | |||
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Revision as of 11:24, 31 May 2012
Materials
| BASAL MEDIUM to make | 1 Liter | 2 Liters | 4 Liters | 5 Liters |
| Glutamic acid, monosodium salt, 1H2O | 10.72g | 21.44g | 42.88g | 53.60g |
| Trisma Base | 1.525g | 3.05g | 6.10g | 7.63g |
| NaCl | 2.50g | 5.00g | 10.00g | 12.50g |
| KH2PO4 | 0.50g | 1.00g | 2.00g | 2.50g |
| KCl | 0.20g | 0.40g | 0.80g | 1.00g |
| MgCl2 6H2O | 100mg | 200mg | 400mg | 500mg |
| CaCl2 2H2O | 20mg | 40mg | 80mg | 100mg |
Procedure
- Add 2.9g of NaCl to a 1 L bottle.
- Add in 25 mL of 7.5 pH Tris-Cl
- Bring this to a volume of 500 mL by adding distilled water.
- Autoclave (large liquid).
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
References
Contact
- Who has experience with this protocol?
or instead, discuss this protocol.
