IGEM:Virginia 2012/Protocols/SSM Media Preparation

Materials

BASAL MEDIUM to make	1 Liter	2 Liters	4 Liters	5 Liters
Glutamic acid, monosodium salt, 1H2O	10.72g	21.44g	42.88g	53.60g
Trisma Base	1.525g	3.05g	6.10g	7.63g
NaCl	2.50g	5.00g	10.00g	12.50g
KH2PO4	0.50g	1.00g	2.00g	2.50g
KCl	0.20g	0.40g	0.80g	1.00g
MgCl2 6H2O	100mg	200mg	400mg	500mg
CaCl2 2H2O	20mg	40mg	80mg	100mg

Procedure

• Add 2.9g of NaCl to a 1 L bottle.
• Add in 25 mL of 7.5 pH Tris-Cl
• Bring this to a volume of 500 mL by adding distilled water.
• Autoclave (large liquid).

Notes

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