Alex Zorychta: /* Overview */

Alex Zorychta — Mon, 04 Jun 2012 16:37:49 GMT

Overview

←Older revision		Revision as of 16:37, 4 June 2012
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	==Overview==		==Overview==

-	Detailed below is the procedure to use the NanoDrop machine to quantify amount of nucleic acid, specifically as prepared from [this protocol].	+	Detailed below is the procedure to use the NanoDrop machine to quantify amount of nucleic acid, specifically as prepared from [http://openwetware.org/wiki/IGEM:Virginia_2012/Protocols/Isolating_the_Phage_Genome this protocol].

	==Materials==		==Materials==

Alex Zorychta: New page: ==Overview== Detailed below is the procedure to use the NanoDrop machine to quantify amount of nucleic acid, specifically as prepared from [this protocol]. ==Materials== * Sterile Water...

Alex Zorychta — Mon, 04 Jun 2012 16:37:09 GMT

New page: ==Overview== Detailed below is the procedure to use the NanoDrop machine to quantify amount of nucleic acid, specifically as prepared from [this protocol]. ==Materials== * Sterile Water...

New page

==Overview==

Detailed below is the procedure to use the NanoDrop machine to quantify amount of nucleic acid, specifically as prepared from [this protocol].

==Materials==

* Sterile Water
* Buffer without sample
* Sample in buffer

==Procedure==

'''Be very careful using the machine, the other lab is very strict about it and misuse may cause us to lose our privilege of using it.'''

* Open up the NanoDrop application.
* Choose "Nucleic Acid" on the GUI.
* Aliquot 2 μL of sterile water on the sample space of the machine and then close it.
* Click "OK." Make sure it clicks as it reads the sample.
* Wipe the sample space with a Kimwipe.
* Next, put in 2 μL of the buffer ''without the sample'' and click "Blank." Wipe it off with a Kimwipe. ''This is our control measurement.''
* Next, put in 2 μL of your sample. Click "Measure." Then clean the sample space with a Kimwipe.
* Lastly, put on 2 μL of sterile water again, press it down, then move it back up again, and wipe it with a Kimwipe.

Name the file, click "Show Report," and "Save Window" into the shared CVRC folder to access it on our own computers.

==Notes==
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!



==References==


==Contact==
*Who has experience with this protocol?

or instead, [[Talk:{{PAGENAME}}|discuss this protocol]].


[[Category:Protocol]]

IGEM:Virginia 2012/Protocols/Using the NanoDrop - Revision history

Alex Zorychta: /* Overview */

Alex Zorychta: New page: ==Overview== Detailed below is the procedure to use the NanoDrop machine to quantify amount of nucleic acid, specifically as prepared from [this protocol]. ==Materials== * Sterile Water...