IGEM:iGEM gto mx/2009/Notebook/Interlab 2016/Entry Base: Difference between revisions
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Objective== | |||
*The objective of this year’s iGEM Interlab study is to quantify expression of five different reporter constructs. These constructs have GFP under the control of different promoter and ribosome binding sequences (RBS). A key development over last year’s study is to evaluate the expression in absolute units. | *The objective of this year’s iGEM Interlab study is to quantify expression of five different reporter constructs. These constructs have GFP under the control of different promoter and ribosome binding sequences (RBS). A key development over last year’s study is to evaluate the expression in absolute units. | ||
== | ==Solutions == | ||
=== For E. coli Transformation === | === For E. coli Transformation === | ||
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{|{{table}} | {|{{table}} | ||
| align="center" style="background:#f0f0f0;"|''' SOC medium''' | | align="center" style="background:#f0f0f0;"|''' SOC medium''' | ||
| align="center" style="background:#f0f0f0;"|''' | | align="center" style="background:#f0f0f0;"|''' ''' | ||
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| Tryptone || 20 g/L | | Tryptone || 20 g/L | ||
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{|{{table}} | |||
| align="center" style="background:#f0f0f0;"|''' LB medium''' | |||
| align="center" style="background:#f0f0f0;"|''' ''' | |||
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| Yeast Extract || 5 g/L | |||
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| NaCl || 5 g/L | |||
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| Casein Peptone (pH 7.2) || 10 g/L | |||
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| Distilled water || 1 L | |||
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| Chloramphenicol 25 mg/mL (dissolve in EtOH) || 25 µg/mL | |||
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==Materials == | |||
=== For E. coli Transformation === | |||
==Protocols == | |||
==Methodology== | |||
==Lab Notebook/log== | |||
==Results and Discussion == | |||
==Conclusions == | |||
==Acknowledgements== | |||
==References == | |||
SIGMA-ALDRICH. SOC Medium S1797 SIGMA For use in transformation. Retrieved from: http://www.sigmaaldrich.com/catalog/product/sigma/s1797?lang=es®ion=MX | |||
PROBIOTEK. CALDO LB (Luria Bertani). Retrieved from: http://www.probiotek.com/producto/caldo-luria-luria-bertani-lb/ | |||
Addgene. Antibiotic Concentrations for Bacterial Selection. Retrieved from: https://www.addgene.org/mol-bio-reference/antibiotics/ | |||
==Notes == | |||
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Latest revision as of 01:52, 27 September 2017
iGEM Project name 1 | Main project page | ||||||||||||||||||||||||||||
Objective
SolutionsFor E. coli Transformation
MaterialsFor E. coli TransformationProtocolsMethodologyLab Notebook/logResults and DiscussionConclusionsAcknowledgementsReferencesSIGMA-ALDRICH. SOC Medium S1797 SIGMA For use in transformation. Retrieved from: http://www.sigmaaldrich.com/catalog/product/sigma/s1797?lang=es®ion=MX PROBIOTEK. CALDO LB (Luria Bertani). Retrieved from: http://www.probiotek.com/producto/caldo-luria-luria-bertani-lb/ Addgene. Antibiotic Concentrations for Bacterial Selection. Retrieved from: https://www.addgene.org/mol-bio-reference/antibiotics/ Notes |