IGEM:iGEM gto mx/2009/Notebook/Interlab 2016/Entry Base

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Objective

  • The objective of this year’s iGEM Interlab study is to quantify expression of five different reporter constructs. These constructs have GFP under the control of different promoter and ribosome binding sequences (RBS). A key development over last year’s study is to evaluate the expression in absolute units.

Solutions

For E. coli Transformation

SOC medium
Tryptone 20 g/L
Yeast Extract 5 g/L
MgSO4 4.8 g/L
Dextrose 3.603 g/L
NaCl 0.5 g/L
KCl 0.186 g/L
Distilled water
LB medium
Yeast Extract 5 g/L
NaCl 5 g/L
Casein Peptone (pH 7.2) 10 g/L
Distilled water 1 L
Chloramphenicol 25 mg/mL (dissolve in EtOH) 25 µg/mL

Materials

For E. coli Transformation

Protocols

Methodology

Lab Notebook/log

Results and Discussion

Conclusions

Acknowledgements

References

SIGMA-ALDRICH. SOC Medium S1797 SIGMA For use in transformation. Retrieved from: http://www.sigmaaldrich.com/catalog/product/sigma/s1797?lang=es&region=MX

PROBIOTEK. CALDO LB (Luria Bertani). Retrieved from: http://www.probiotek.com/producto/caldo-luria-luria-bertani-lb/

Addgene. Antibiotic Concentrations for Bacterial Selection. Retrieved from: https://www.addgene.org/mol-bio-reference/antibiotics/

Notes


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