IPTG: Difference between revisions
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Isopropyl-beta-D-thiogalactopyranoside | Isopropyl-beta-D-thiogalactopyranoside | ||
Induces transcription from promoters regulated by | Induces transcription from promoters regulated by LacI repressor. | ||
Molecular weight is 238.31 g/mol | Molecular weight is 238.31 g/mol. The chemical formula is [http://www.fermentas.com/catalog/reagents/img/iptg.gif here]. | ||
#Dissolve 1g in 4196 μL deionized water to make 1M solution. | #Dissolve 1g in 4196 μL deionized water to make 1M solution. | ||
#Filter sterilize with syringe and 0.22μm filter | #Filter sterilize with syringe and 0.22μm filter | ||
Alternatively | |||
#Dissolve 238 mg IPTG (sigma I-6758) in 10 ml DW for (100mM stock) | |||
#store in 1 ml aliquotes at -20°C | |||
Generally a 1mM solution is an effective amount to induce the pLac promoter region. It should be noted that this may vary over cell strains. For example: | Generally a 1mM solution is an effective amount to induce the pLac promoter region. It should be noted that this may vary over cell strains. For example: | ||
*lacIq: cell strains which overproduce | *lacIq: cell strains which overproduce LacI repressor (ie. [[E. coli genotypes|''E. coli'' cell strain]] type [[E. coli genotypes#D1210|D1210]], aka. [[registry:Part:BBa_V1003|BBa_V1003]]) | ||
For including IPTG in LB agar plates, a typical amount people recommend is 0.1-0.5mM IPTG. | |||
[[Category:Material]] [[Category:Chemical]] | |||
Latest revision as of 14:26, 23 December 2011
Isopropyl-beta-D-thiogalactopyranoside
Induces transcription from promoters regulated by LacI repressor.
Molecular weight is 238.31 g/mol. The chemical formula is here.
- Dissolve 1g in 4196 μL deionized water to make 1M solution.
- Filter sterilize with syringe and 0.22μm filter
Alternatively
- Dissolve 238 mg IPTG (sigma I-6758) in 10 ml DW for (100mM stock)
- store in 1 ml aliquotes at -20°C
Generally a 1mM solution is an effective amount to induce the pLac promoter region. It should be noted that this may vary over cell strains. For example:
- lacIq: cell strains which overproduce LacI repressor (ie. E. coli cell strain type D1210, aka. BBa_V1003)
For including IPTG in LB agar plates, a typical amount people recommend is 0.1-0.5mM IPTG.
