IPTG: Difference between revisions
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#Filter sterilize with syringe and 0.22μm filter | #Filter sterilize with syringe and 0.22μm filter | ||
Generally a 1mM solution is an effective amount to induce the pLac promoter region. | Generally a 1mM solution is an effective amount to induce the pLac promoter region. It should be noted that this may vary over cell strains. For example: | ||
*lacIq: cell strains which overproduce lacI repressor (ie. [[E. coli genotypes|E.coli cell strain]] type [[http://openwetware.org/wiki/E._coli_genotypes#D1210|D1210]], aka. [http://parts.mit.edu/registry/index.php/Part:BBa_V1003 BBa_V1003]) | |||
Revision as of 12:48, 17 July 2006
Isopropyl-beta-D-thiogalactopyranoside
Induces transcription from promoters regulated by lacI repressor.
Molecular weight is 238.31 g/mol
- Dissolve 1g in 4196 μL deionized water to make 1M solution.
- Filter sterilize with syringe and 0.22μm filter
Generally a 1mM solution is an effective amount to induce the pLac promoter region. It should be noted that this may vary over cell strains. For example:
- lacIq: cell strains which overproduce lacI repressor (ie. E.coli cell strain type [[1]], aka. BBa_V1003)
