IPTG: Difference between revisions
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Induces transcription from promoters regulated by lacI repressor. | Induces transcription from promoters regulated by lacI repressor. | ||
Molecular weight is 238.31 g/mol | Molecular weight is 238.31 g/mol. The chemical formula is [http://www.fermentas.com/catalog/reagents/img/iptg.gif here]. | ||
#Dissolve 1g in 4196 μL deionized water to make 1M solution. | #Dissolve 1g in 4196 μL deionized water to make 1M solution. | ||
Revision as of 08:09, 14 August 2007
Isopropyl-beta-D-thiogalactopyranoside
Induces transcription from promoters regulated by lacI repressor.
Molecular weight is 238.31 g/mol. The chemical formula is here.
- Dissolve 1g in 4196 μL deionized water to make 1M solution.
- Filter sterilize with syringe and 0.22μm filter
Generally a 1mM solution is an effective amount to induce the pLac promoter region. It should be noted that this may vary over cell strains. For example:
- lacIq: cell strains which overproduce lacI repressor (ie. E.coli cell strain type D1210, aka. BBa_V1003)
