ISISBio:Internal/Group meetings/13-June-08: Difference between revisions

Latest revision as of 04:31, 13 June 2008

Safety Issues:

Autoclave is currently being quoted for plumbing in which seems to be getting out of hand from a cost perspective.
Up to two days disruption to fit tank in ceiling
Query over status of bunsen burner, some suggestion that it needs to be protected and clamped down. This isn't really practical.
Use of chloroform to clean troughs
- Action LW to ask for risk assessment for trough use

Current Projects Status:

Preparation of protein for QENS
- MT - lots of GFP available, needs to be purified by gel filtration in advance of beamtime
- Need to not spend too much more time on this
  - Action CN to confirm with MT when the beamtime is, prefer September
Preparation of mellitin for VGS
- Currently checking to see whether initial plasmid has been made
- Several more mol biol steps to go
- Action LW to follow through with next steps
OmpF for SAH
- LC has purified significant quantities of OmpF, total of about 6 mg
- Played around with making vesicles. PE doesn't make very good vesicles so need to move 25:25:50 PE:PG:PC vesicles
- Going to Oak Ridge for two weeks from 29 June -9 July to deuterate OmpF for SANS experiments
- May need to 5mL DEAE column to Oak ridge for final wash stage
- External reflection IR and DLS organised to do at Reading
Ion channels
- Currently trying to purify MLok1 in large quantities for ILL on 19 July
- Recent issues with funny bands on gels which seemed to appear in last two weeks. Checking batches against each other.
- Some sample to take but possibly not as much as we want. Would like 3mg/mL for 5/6 contrasts.
- Will also take some mthK to ILL to see whether can see change on addition of Ca2+
Fluorescent labelling
- Think Michael had made pcr product,now need to purify and ligate to make plasmid

Dave Barlow/Damian experiment to express and purify GlpF/DCytB
- Action CN to contact DB and suggest moving schedule up to avoid Thursday