ISISBio:Internal/Lab Supplies

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Required items

• GE Healthcare XK 16/20 column 18-8773-01
• GE Healthcare HiPrep 16/10 DEAE-FF 17-5090-01

Ordered items

• 10/03/2008
• Sigma low range molecular weight markers Sigma 6.5 - 66 kDa M3913-10vl x1
• Sigma Mid range molecular weight markers Sigma 6.5 - 200 kDa S8445-1vl x4
• Sigma high range molecular weight markers Sigma 36 - 20 kDa S8320-10vl x1
• Sample buffer solution Sigma S3401-10vl x2
• LDao (n,n-Dimethyldodecylamine n-oxide) Sigma D9775-SG x1

• 07/03/2008
• n-dodecyl beta-D-maltose D4641-SG - Merck
• cAMP
• BL21 (DE3) competent cells
• 2 x 100g electrophoresis agarose - Fisher
• 3kg balance - Fisher
• 1Kg Ammonium Acetate - Fisher
• 0.5ul-10ul tips 1000pk - Fisher
• 1ul-200ul tips 1000pk - Fisher
• 250ul-1000ul tips 1000pk - Fisher

• GEHealthcare - PEEK tubing 1/16", i.d. 0.75 mm 2 M 18-1112-53 1 £50.50 £50.50
• Superloop, 1/16" fittings (ÄKTAdesign) 10 ml 1 EA 18-1113-81 1 £360.00 £360.00 Sub Total: £410.50

Items arrived

• 3.5 KDa cut off dialysis tubing
• PCR thermocycle control panel
• Miniplasmid kits
• Acetic acid
• DTT
• glycine
• sodium bicarbonate
• EDTA
• chloramphenicol
• butane cartridges for bunsen
• ethidium bromide
• kanamycin sulphate
• ampicillin
• 0.45 u sterile filters

20 February

• 1000 MWCO Spectrapor dialysis tubing
• ammonium acetate
• lysozyme

25 Febuary=

• Yeast extract
• 0.22 um u sterile filters
• X-gal
• KCl
• KH2Phos
• Na2Hphos
• Triton X-100
• biohazard autoclave gloves
• bunsen burner
• batteries
• orange bins
• SDS

4 march

• Top 10 cells
• Arabinose