ISISBio:Internal/Lab Supplies: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 4: | Line 4: | ||
The following to be ordered by Louise | The following to be ordered by Louise | ||
* DNA marker (e.g. Fisher BPE2553-100) | |||
* A4 scanner with one button scanning | * A4 scanner with one button scanning | ||
* UV lamp for cutting gels | * UV lamp for cutting gels | ||
| Line 14: | Line 15: | ||
* Containers for agar plates | * Containers for agar plates | ||
* Shelves from Screw fix x2 | * Shelves from Screw fix x2 | ||
==Wish List== | |||
* Benchtop centrifuge suitable for 50 mL Falcon tubes | |||
==Ordered items== | ==Ordered items== | ||
Revision as of 08:12, 20 March 2008
Required items
The following to be ordered by Louise
- DNA marker (e.g. Fisher BPE2553-100)
- A4 scanner with one button scanning
- UV lamp for cutting gels
- GE Healthcare XK 16/20 column 18-8773-01
- GE Healthcare HiPrep 16/10 DEAE-FF 17-5090-01
- Jencons 412-167 spot labels
- containers to transport durans to and from balance autoclave
- Containers for agar plates
- Shelves from Screw fix x2
Wish List
- Benchtop centrifuge suitable for 50 mL Falcon tubes
Ordered items
Items arrived
20 February
- 1000 MWCO Spectrapor dialysis tubing
- ammonium acetate
- lysozyme
25 Febuary=
- Yeast extract
- 0.22 um u sterile filters
- X-gal
- KCl
- KH2Phos
- Na2Hphos
- Triton X-100
- biohazard autoclave gloves
- bunsen burner
- batteries
- orange bins
- SDS
4 march
- Top 10 cells
- Arabinose
10 march
- 2 x 100g electrophoresis agarose - Fisher
- 1Kg Ammonium Acetate - Fisher
- 1ul-200ul tips 1000pk - Fisher
- 250ul-1000ul tips 1000pk - Fisher
- 3.5 KDa cut off dialysis tubing
- PCR thermocycle control panel
- Miniplasmid kits
- Acetic acid
- DTT
- glycine
- sodium bicarbonate
- EDTA
- chloramphenicol
- butane cartridges for bunsen
- ethidium bromide
- kanamycin sulphate
- ampicillin
- 0.45 u sterile filters
11 March
- ordered 10/03/2008
- Promega Wizard Gel and PCR Clean up Kit 250rx A9282 x1
- GEHealthcare - PEEK tubing 1/16", i.d. 0.75 mm 2 M 18-1112-53 1 £50.50 £50.50
- Superloop, 1/16" fittings (ÄKTAdesign) 10 ml 1 EA 18-1113-81 1 £360.00 £360.00 Sub Total: £410.50
- n-dodecyl beta-D-maltose D4641-SG - Merck
- cAMP-Merck
- BL21 (DE3) competent cells -Merck
12th March
- Sigma low range molecular weight markers Sigma 6.5 - 66 kDa M3913-10vl x1
- Sigma Mid range molecular weight markers Sigma 6.5 - 200 kDa S8445-1vl x4
- Sigma high range molecular weight markers Sigma 36 - 20 kDa S8320-10vl x1
- Sample buffer solution Sigma S3401-10vl x2
- LDao (n,n-Dimethyldodecylamine n-oxide) Sigma D9775-SG x1
13th March
- ordered 12/03/2008
- T4 Polynucleotide kinase - recombinant from E.Coli Fisher BioReagents 100 U BPE3212-1 £23.50 Fisher Scientific
- PCR tube Fisherbrand thin walled domed cap polypropylene 0.2mL natural pack of 1000 FB68720 £64.60 Fisher Scientific x2
These inc VAT £131.55 Ref.7482996 Gail x2234
- ordered 11/03/2008
- Fisher GHL-415-010D gas burner lighter x2
- Fisher FB67020 pH Electrode x1
- Fisher FB70260 Timer x6
- Fisher BMD-110-021W Batteries x6
- Fisher LAB-550-010W white tape x1
- Fisher LAB-551-010Q Orange tape x1
- Fisher LAB-553-010E Red tape x1
- Fisher LAB-555-010E Yellow tape x1
- Fisher LAB-557-010D Green tape x1
- Fisher LAB-559-010X Blue tape x1
- Fisher LAC-730-010K Black pen 10pk x1
- Fisher LAC-730-020H Blue pen 10pk x1
- Fisher LAB-340-030Q Round labels 1000pk x1
- Fisher HW1-LB-S small lab coat x9
- Fisher HW1-LB-M Medium lab coat x9
- Fisher FB55301 5pk Clamp set
- Fisher FB68403 Base for clamp x2
- Fisher FB55305 Bosshead Clamping screws 5pk x1
- Fisher FB34531 1-200ul tips 1000pk x10
- Fisher FB34511 0.5ul-10ul tips 1000pk x10
- Fisher FB34611 200-1000ul tips 1000pk x10
- Fisher FB70173 cleaning detergent
- Total of order inc VAT £1367.22
- ordered 07/03/2008
- 3kg balance - Fisher
- 0.5ul-10ul tips 1000pk - Fisher
