ISISBio:Internal/Lab Supplies

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Fri 11th April 2008

• get in an autoclave engineer - will do Mon a.m.
• continue with Vicki's work - will do Mon a.m.
• find out about centrifuges, rotors and protein concentrators
• look into cover to prevent condensation dripping on AKTAs in cold room
• move fermentor & control unit to Milton Park
• Put a sign in cupboard to say NO LAB COATS ALLOWED IN HERE
• Find out about booking system for Biolab
• Make a back page here and put old arrived items list in it
• Reply to last PC email and enquire about rest of order
• Ask Matt about benching for BAM room and Biolab - done this - pending receipt of quotes & authorisation to place order
• Sort out ELGA systems in Biolab - Matt will do next week
• Work out costings for workers/users of Biolab
• Prepare log sheets for Biolab equipment to be used for bookings
• Do RAs and COSHH A in Biolab starting with updating Biolab general room RA, then autoclave RA.
• Get in engineer for validations of autoclave and centrifuge commissioning - am chasing this on Mon
• Chase Matt about ELGA engineer - chasing on Mon
• Complete APR - start this next Friday
• Chase up Matt for shaker incubator warranty/service info
• do same for fermentor

For Cameron to do:

• Show Louise how to make a back page from this lab supplies one
• Follow up what is happening with the trough
• Ask Matt about organising test build of light scattering apparatus to go into Vesta

Required items

The following to be ordered by Louise

• A4 scanner with one button scanning - Cameron to sort out brand or issues
• Jencons 412-167 spot labels
• -20 freezer
• Furniture for Biolab and BAM room
• Wire baskets
• -80 freezer storage-fisher
• Freezer boxes
• Petridish rack
• Order fume hood & cupboard
• polycarbonate centrifuge tubes
• dodecyl maltoside 5g
• weighing boats
• large tape dispenser for coloured tapes

Wish List

• Benchtop centrifuge suitable for 50 mL Falcon tubes

Ordered items

11th April 2008

Fisher order 7515525 1104081

• 10pk 500ml beakers
• 2 x 5pk 1L beakers
• 10pk 250ml beakers ordered but out of stock til May!
• 10pk 100ml glass beaker
• 10pk 250ml glass beaker
• 10pk 600ml glass beaker
• 3x 1L glass beaker
• 2 x 1000pk PCR tubes 0.2ml
• 1.5ml tube float rack 4pk
• 0.5ml tube float rack 4pk
• 25g low melting agarose
• 5 thermometers -10 to 110 degrees
• 0.5ul-10ul tips 10 x 1000pk ordered but out of stock til May!

7th April 2008

Screw Fix

• 3 shelf units for cold room

2nd April 2008

• Fisher Incubator Shelf & 2 Supports x2 ING-205-502L

Week 2

11th April 2008

• ELGA cartridges LC148, LC163, LC181, CT all x2

10th April 2008

• Steps/ladders from screw fix

9th April 2008

• Fisher 100 mg anhydrotetracycline (not Louise's GPC)

8th April 2008

Screw Fix

• Trolley for lab move and/or fermentor
• Spirit level for lab
• 6 containers for transporting bottles across lab
• Mobile step for office

Week1

3rd April 2008

• Fisher 5L Beakers x5 FB33119 £11.74 each
• Fisher Magnetic Retriever x1 FB55669
• Fisher Stirrer fleas 10pk x1 FB55601
• Fisher Stirrer fleas 10pk x1 FB55591

MARCH

31th March 2008

ordered 26/03/08

• BPE2543-2 Thrombin, bovine plasma Fisher BioReagents from Fisher Scientific
• Merck- n-Dodecyl-b-D-maltoside, ULTROL® Grade Cat. No. 324355 5g

28th March 2008

ordered 27/03/08

• Fisher- Protein Concentrators 2ml 10KMW Millipore
• Fisher- Containers for agar plates

27th March 2008

• Ultra violet DNA gel lamp and spares
• DNA marker (e.g. Fisher BPE2553-100)
• GE Healthcare XK 16/20 column 18-8773-01
• GE Healthcare HiPrep 16/10 DEAE-FF 17-5090-01

20th March 2008

• ordered 07/03/2008
• 3kg balance - Fisher
• 0.5ul-10ul tips 1000pk - Fisher

13th March

• ordered 12/03/2008
• T4 Polynucleotide kinase - recombinant from E.Coli Fisher BioReagents 100 U BPE3212-1 £23.50 Fisher Scientific
• PCR tube Fisherbrand thin walled domed cap polypropylene 0.2mL natural pack of 1000 FB68720 £64.60 Fisher Scientific x2

These inc VAT £131.55 Ref.7482996 Gail x2234

• ordered 11/03/2008
• Fisher GHL-415-010D gas burner lighter x2
• Fisher FB67020 pH Electrode x1
• Fisher FB70260 Timer x6
• Fisher BMD-110-021W Batteries x6
• Fisher LAB-550-010W white tape x1
• Fisher LAB-551-010Q Orange tape x1
• Fisher LAB-553-010E Red tape x1
• Fisher LAB-555-010E Yellow tape x1
• Fisher LAB-557-010D Green tape x1
• Fisher LAB-559-010X Blue tape x1
• Fisher LAC-730-010K Black pen 10pk x1
• Fisher LAC-730-020H Blue pen 10pk x1
• Fisher LAB-340-030Q Round labels 1000pk x1
• Fisher HW1-LB-S small lab coat x9
• Fisher HW1-LB-M Medium lab coat x9
• Fisher FB55301 5pk Clamp set
• Fisher FB68403 Base for clamp x2
• Fisher FB55305 Bosshead Clamping screws 5pk x1
• Fisher FB34531 1-200ul tips 1000pk x10
• Fisher FB34511 0.5ul-10ul tips 1000pk x10
• Fisher FB34611 200-1000ul tips 1000pk x10
• Fisher FB70173 cleaning detergent
• Total of order inc VAT £1367.22

12th March

• Sigma low range molecular weight markers Sigma 6.5 - 66 kDa M3913-10vl x1
• Sigma Mid range molecular weight markers Sigma 6.5 - 200 kDa S8445-1vl x4
• Sigma high range molecular weight markers Sigma 36 - 20 kDa S8320-10vl x1
• Sample buffer solution Sigma S3401-10vl x2
• LDao (n,n-Dimethyldodecylamine n-oxide) Sigma D9775-SG x1
• Spatulas 0.09inch tip Sigma Z243213-2EA £11.80 x10

11 March

• ordered 10/03/2008
• Promega Wizard Gel and PCR Clean up Kit 250rx A9282 x1
• GEHealthcare - PEEK tubing 1/16", i.d. 0.75 mm 2 M 18-1112-53 1 £50.50 £50.50
• Superloop, 1/16" fittings (ÄKTAdesign) 10 ml 1 EA 18-1113-81 1 £360.00 £360.00 Sub Total: £410.50
• n-dodecyl beta-D-maltose D4641-SG - Merck
• cAMP-Merck
• BL21 (DE3) competent cells -Merck

10 march

• 2 x 100g electrophoresis agarose - Fisher
• 1Kg Ammonium Acetate - Fisher
• 1ul-200ul tips 1000pk - Fisher
• 250ul-1000ul tips 1000pk - Fisher
• 3.5 KDa cut off dialysis tubing
• PCR thermocycle control panel
• Miniplasmid kits
• Acetic acid
• DTT
• glycine
• sodium bicarbonate
• EDTA
• chloramphenicol
• butane cartridges for bunsen
• ethidium bromide
• kanamycin sulphate
• ampicillin
• 0.45 u sterile filters

4 march

• Top 10 cells
• Arabinose

FEBRUARY

25 Febuary=

• Yeast extract
• 0.22 um u sterile filters
• X-gal
• KCl
• KH2Phos
• Na2Hphos
• Triton X-100
• biohazard autoclave gloves
• bunsen burner
• batteries
• orange bins
• SDS

20 February

• 1000 MWCO Spectrapor dialysis tubing
• ammonium acetate
• lysozyme