Identifying genes and molecular machines that drive root hair tip growth: Difference between revisions
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==T-DNA insertion mutant screen== | |||
In order to assess putative functions of the candidate genes in the root hair transcriptome | |||
<br/><br/><br/><br/><br/>[[Grierson Lab|Grierson Lab homepage]] [[Grierson Lab:Back Door|Lab Members]] | <br/><br/><br/><br/><br/>[[Grierson Lab|Grierson Lab homepage]] [[Grierson Lab:Back Door|Lab Members]] |
Revision as of 07:17, 14 September 2006
Creation of a root hair tip growth transcriptome
By combining two independent microarray experiments comparing hairless roots and roots with root hairs, a candidate list of 150 genes that are statistically upregulated in root hair cells has been created. The table below represents all the functional categories that are overrepresented in the tip growth transcriptome compared to the genome. It includes many functions that are essential for rapid cell growth.
Downregulated genes in root hair growth encode putative stress reponse genes
The table below summarises the overrepresented gene functions in the 86 genes downregulated in root hairs during tip growth. These include responses to external stimuli, which could possibly be due to the cell focusing more cellular resources on tip growth at the expense of defence. A suppression of genes that react to ROS is also evident, which may be required due to the abnormally high levels of ROS seen in root hairs. Unsurprisingly flavonoid biosynthesis, which acts as blue/red pigment in flowers (and sporadically as antioxidants), is downregulated in root hairs.
T-DNA insertion mutant screen
In order to assess putative functions of the candidate genes in the root hair transcriptome
Grierson Lab homepage Lab Members