Immunocytochemistry

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=Immunocytochemistry=
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Immunocytochemistry (ICC) is the process of taking tissue culture cells and applying antibodies to determine the spatial and temporal features of a particular antigen (often protein).  A survey of the literature reveals many different techniques.  However, there is often very little comment about the individual steps of the techniques, and hopefully this page will lead to a productive discussion.  Often different techniques applied to the same antibody will reveal different results. 
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Immunofluorescence (IF) is a subset of ICC in which the antibody localization is visualized by fluorescence.
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