Immunocytochemistry

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=Introduction=
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Immunocytochemistry (ICC) is the process of taking tissue culture cells and applying antibodies to determine the spatial and temporal features of a particular antigen (often protein).  A survey of the literature reveals many different techniques.  However, there is often very little comment about the individual steps of the techniques, and hopefully this page will lead to a productive discussion.  Often different techniques applied to the same antibody will reveal different results. 
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Immunofluorescence (IF) is a subset of ICC in which the antibody localization is visualized by fluorescence.  The below protocol is focused on indirect IF (the use of a secondary antibody).
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=The Seven Basic Steps=
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# Grow Cells
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# Fix Cells
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# Permabalize Cells
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# Block Cells
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# Incubate with Primary Antibody
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# Incubate with Secondary Antibody
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# Place on a slide
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==Grow Cells==
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The key point to be made here is to not have too many or too few cells on the day you harvest the cells.
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