Immunocytochemistry

Immunocytochemistry

Immunocytochemistry (ICC) is the process of taking tissue culture cells and applying antibodies to determine the spatial and temporal features of a particular antigen (often protein). A survey of the literature reveals many different techniques. However, there is often very little comment about the individual steps of the techniques, and hopefully this page will lead to a productive discussion. Often different techniques applied to the same antibody will reveal different results.

Immunofluorescence (IF) is a subset of ICC in which the antibody localization is visualized by fluorescence.