Individual Journal Week 11: Difference between revisions
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===Outline=== | ===Outline=== | ||
==Introduction== | ===Introduction=== | ||
*The study observes the effects of suboptimal temperatures on transcriptional regulation in yeast. | *The study observes the effects of suboptimal temperatures on transcriptional regulation in yeast. | ||
*Transcriptional responses were observed at temperatures 12C and 30C. | *Transcriptional responses were observed at temperatures 12C and 30C. |
Revision as of 20:12, 3 April 2013
Terms
- Batch-culture: A large-scale closed system culture in which cells are grown in a fixed volume of nutrient culture medium under specific environmental conditions (e.g. nutrient type, temperature, pressure, aeration, etc.) up to a certain density in a tank or airlift fermentor, harvested and processed as a batch, especially before all nutrients are used up. [1]
- Catabolite: product of catabolism, the breakdown of complex molecules into simpler ones. [2]
- Gene Regulatory Protein: Any protein that interacts with dna sequences of a gene and controls its transcription. [3]
- Up-regulation:process that increases ligand/receptor interactions due to an increase in the number of available receptors. [4]
- Down-regluation: the process that decreases ligand and receptor interactions or reduces the responsiveness of a cell to a stimulus following first exposure.This is often accompanied by an initial decrease in affinity of receptors for the agent and a subsequent reduction in the number of available receptors expressed on the surface which can result from internalisation of the ligand:receptor complex or from decreased expression of the receptor. [5]
- Motif: The smallest group of atoms in a polymer that, when under the influence of a rotation-translation operator, will assemble the rest of the atoms in the chain. [6]
- Desaturase: Any of several enzymes that putdouble bonds into the hydrocarbon areasof fatty acids. [7]
- Biogenesis: The process in which life forms arise from similar life forms. [8]
- Trehalose: a crystalline disaccharide C12H22O11 that is found in various organisms (as fungi and insects), is about half as sweet as sucrose, and is sometimes used as a sweetener in commercially prepared foods [9]
- Transcriptome: the complement of mature messenger RNAs produced in a given cell in a given moment of its life. [10]
Outline
Introduction
- The study observes the effects of suboptimal temperatures on transcriptional regulation in yeast.
- Transcriptional responses were observed at temperatures 12C and 30C.
- Lower temperatures, typically result in the slower cellular processes: growth phase, respiration, lipid composition of membranes, and trehalose content.
- Time scale of exposure to cold-shock relevant:
- Sudden exposure => adaptation
- Prolonged exposure=> acclimation
- Long term exposure => evolutionary adaptation
- Two distinct phases during cold-shock response
- First 12 hours, early cold shock (ECS)
- After first 12 hours, Late cold response (LCR)
- TPS1 and TPS2, commonly observed trehalose-biosynthetic transcriptional induction genes- observed in cold and heat shock conditions
- Genes involved in cold-shock response typically involved in other stress response
- Growth rate an important factor on transcription
- Research seeks to control specific growth rate and other culture conditions, through use of chemostat, in order to establish steady-state and, thus, investigate transcription regulation influenced by suboptimal temperatures