Instructions: Difference between revisions
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'''2)''' Remove the coomassie stain (can be reused), and replace with destaining solution for 30 minutes<br> | '''2)''' Remove the coomassie stain (can be reused), and replace with destaining solution for 30 minutes<br> | ||
'''3)''' Remove the destaining solution (into waste bottle) and replace with destaining solution for 30 minutes-overnight<br> | '''3)''' Remove the destaining solution (into waste bottle) and replace with destaining solution for 30 minutes-overnight<br> | ||
'''4)''' If desired, repeat the destaining process | '''4)''' If desired, repeat the destaining process<br> | ||
'''5)''' When finished destaining, leave the gel in water. If desired preserve the gel in cellophane. | '''5)''' When finished destaining, leave the gel in water. If desired preserve the gel in cellophane. | ||
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'''Staining an agarose gel with ethidium bromide'''<br> | |||
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'''1)''' After electrophoresis place the gel into the staining solution for 15 minutes with mild shaking | |||
'''2)''' Place the gel into water and visualize under a UV lamp<br> | |||
Revision as of 17:56, 12 March 2009
Staining a SDS-PAGE gel with coomassie blue
1) Cover the gel with coomassie staining solution and leave on a shaker at slow speed for 30 minutes
2) Remove the coomassie stain (can be reused), and replace with destaining solution for 30 minutes
3) Remove the destaining solution (into waste bottle) and replace with destaining solution for 30 minutes-overnight
4) If desired, repeat the destaining process
5) When finished destaining, leave the gel in water. If desired preserve the gel in cellophane.
Staining an agarose gel with ethidium bromide
1) After electrophoresis place the gel into the staining solution for 15 minutes with mild shaking
2) Place the gel into water and visualize under a UV lamp
