Integrases/Excisionases pairs to test: Difference between revisions
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[http://www.ncbi.nlm.nih.gov/pubmed/11123671?ordinalpos=14&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum [1]] ''Genome organization and characterization of mycobacteriophage Bxb1.'', mediavilla et al., Mol microbiol, 2000; PMID 1123671. |
Revision as of 16:39, 13 January 2009
The first experiment is design to test if integrases and excisionases pairs can flip a target sequence in both directions in a controlled manner. Then, we will test if these integrases are specific by putting them in presence of non-target sites. If this first set of experiment is successfull, we will thn perform a kinetic analysis of flipping, by varying time of induction and concentrations of recombinases.
Starter
Integrases/Excisionases pairs
We will start to test 3 pairs of the serine integrase family. Integrases from this family are interesting because they do not require specific cofactors and have been shown to work in a wide range of species. These 3 pairs are the best characterized members from the family which have an identified excisionase.
- Bxb1
From Mycobacterium phage Bxb1.
Uniprot entry:
Bxb1 integrase Bxb1 excisionase
References:
[1] Genome organization and characterization of mycobacteriophage Bxb1., mediavilla et al., Mol microbiol, 2000; PMID 1123671.