Integrases/Excisionases pairs to test: Difference between revisions
| Line 29: | Line 29: | ||
Uniprot entries: | Uniprot entries: | ||
[http://www.uniprot.org/uniprot/ q38184 TP901-1 integrase] [http://www.uniprot.org/uniprot/ o48506 TP901-1 excisionase] | [http://www.uniprot.org/uniprot/q38184 TP901-1 integrase] [http://www.uniprot.org/uniprot/o48506 TP901-1 excisionase] | ||
References: | |||
* | * phiRV1 [int: o06604, RDF: o06606] | ||
From | From | ||
| Line 40: | Line 41: | ||
Uniprot entries: | Uniprot entries: | ||
[http://www.uniprot.org/uniprot/ | [http://www.uniprot.org/uniprot/o06604 phiRV1 integrase] [http://www.uniprot.org/uniprot/o06606 phiRV1 excisionase] | ||
Revision as of 16:53, 13 January 2009
The first experiment is design to test if integrases and excisionases pairs can flip a target sequence in both directions in a controlled manner. Then, we will test if these integrases are specific by putting them in presence of non-target sites. If this first set of experiment is successfull, we will thn perform a kinetic analysis of flipping, by varying time of induction and concentrations of recombinases.
Starter
Integrases/Excisionases pairs
We will start to test 3 pairs of the serine integrase family. Integrases from this family are interesting because they do not require specific cofactors and have been shown to work in a wide range of species. These 3 pairs are the best characterized members from the family which have an identified excisionase.
- Bxb1
From Mycobacterium phage Bxb1.
Uniprot entry:
Bxb1 integrase Bxb1 excisionase
References:
[1] Genome organization and characterization of mycobacteriophage Bxb1., mediavilla et al., Mol microbiol, 2000. PMID 1123671.
[2] Control of phage Bxb1 excision by a novel recombination directionality factor., Ghosh P. et al, PLoS Biol., 2006. PMID: 16719562.
[3] Phage Bxb1 integrase mediates highly efficient site-specific recombination in mammalian cells., Russel JP et al., Biotechniques, 2006. PMID:16629393
- TP901-1
From Lactococcus lactis phage TP901-1.
Uniprot entries:
TP901-1 integrase TP901-1 excisionase
References:
- phiRV1 [int: o06604, RDF: o06606]
From
Uniprot entries:
