J'aime C. Moehlman's Week 11: Difference between revisions
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What do they say about how they performed each of the steps listed in the Overview of Microarray Data Analysis section above? | What do they say about how they performed each of the steps listed in the Overview of Microarray Data Analysis section above? | ||
Briefly state the result shown in each of the figures and tables. | Briefly state the result shown in each of the figures and tables. | ||
How do the results of this study compare to the results of previous studies (See Discussion). | How do the results of this study compare to the results of previous studies (See Discussion). | ||
====Figures==== | ====Figures==== | ||
*Figure 1: | *Figure 1: | ||
*Figure 2: | *Figure 2: | ||
Revision as of 10:57, 6 April 2010
Microarray Journal Club Assignment
Vibrio cholerae Journal Article: Merrell DS, Butler SM, Qadri F, Dolganov NA, Alam A, Cohen MB, Calderwood SB, Schoolnik GK, and Camilli A. Host-induced epidemic spread of the cholera bacterium. Nature 2002 Jun 6; 417(6889) 642-5. doi:10.1038/nature00778
Terms & Definitions
- Chemotaxis: A response of motile cells or organisms in which the direction of movement is affected by the gradient of a diffusible substance, the gradient on which diffusion occurs is in one direction
- Murine: Of, or relating to, a member of the rodent family muridae, including rats and mice.
- Gavage: Therapeutic use of a high-potency diet administered by stomach tube.
- lacZ gene: A gene that encodes for beta-galactosidase that is a lactase which cleaves lactose.
- Homogenization:The process by which a material is made homogeneous, which means; consisting of or composed of similar elements or ingredients, of a uniform quality throughout.
- In- vitro: Processes or reactions that would normally occur within an organism but are made to occur in an artificial environment; such as a test tube, beaker, etc.
- Dissemination: The act/ property of dispersing or diffusing something.
- Hypervirulent: A high quantity, above normal or a high state or degree of something that is extremely toxic, denoting a markedly pathogenic microorganism.
- Electrophoresis: Separation of ionic molecules, (principally proteins) by the differential migration through a gel according to the size and ionic charge of the molecules in an electrical field. High resolution techniques normally use a gel support for the fluid phase.
- Regulon: A collection of genes or operons under regulation by the same regulatory protein.
- Transcriptome: The set of all RNA molecules, including mRNA, rRNA, tRNA, and non-coding RNA produced in one or a population of cells and the transcriptome can vary with external environmental conditions.
Definitions from: biology-online.org dictionary
Article Outline
- The main result presented in the paper
What is the main result presented in this paper? (Hint: look at the last sentence of the introduction and restate it in plain English.) What is the importance or significance of this work? What were the limitations in previous studies that led them to perform this work? What were the methods used in the study? What samples did they collect and use for the microarray experiment? How many microarray chips did they hybridize in the experiment? Which samples were paired to hybridize on the chip? Which was labeled red (Cy5)? Which was labeled green (Cy3)? How many replicates did they perform of each type? Biological replicates are made from entirely different biological samples. Technical replicates are made when one biological sample is split at a particular stage in the procedure and then carried through to the end of the procedure. What do they say about how they performed each of the steps listed in the Overview of Microarray Data Analysis section above? Briefly state the result shown in each of the figures and tables. How do the results of this study compare to the results of previous studies (See Discussion).
Figures
- Figure 1:
- Figure 2:
