J'aime C. Moehlman's Week 2

• Week 2 Assignment

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BIOL398-01/S10

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In order to determine the inherited colors in the flowers we started out by crossing green-1 and a white flowers.

Objectives

• Produce a true-breeding purple flower by using the Aipotu program.
• Determine what alleles are dominant or recessive.

Observations

• When crossing a pure breeding green flower with a pure breeding white flower all of the offspring produced were green. Green is the dominent allele and in this first test-cross the white flowers are the recessive allele.
• When I self crossed flower 1-12 from my first cross, 3 white flowers appeared in 2-1, 2-10,and 2-20; this shows that the green flower I chose was a heterozygous flower.
• Ran 2 mutation trials with 2-10 and 2-20 and the results were all white offspring with both. After mutating 2-1 there was a difference, 2-1 produced a single red flower.
• Self-crossed the red flower (6-6) that resulted from mutating the white flower in 2-1 and the result was that the dominant flower was the red one in this case, however some whites were produced.
• Mutated the red mutated flower (6-6) and the result ended up with many red flowers, 2 white flowers, and one orange flower(8-5).
• After self crossing the orange flower, it produced yellow, orange, and white flowers.
• I added the first orange flower to my greenhouse.
• Crossed orange mutant with green 2 and produced a new purple flower.
• crossed green 2 and purple from above and produced blue, orange, green, and purple flowers.
• crossed blue and purple from greenhouse and produced ONLY blue and purple offspring.
• crossing purple and red creates purple, red, and blue offspring.
• Blue is the dominant flower when self-crossed.
• green-1 only produces green when crossed with anything.
• green-2 self cross produces green, yellow, and blue flowers; this shows incomplete dominance.

Methods

The methods used in this lab, was that of trial and error as well as using tables to cross different flowers and determine the genotypes and phenotypes of the offspring produced.

Aipotu Task Answers

1. How many different alleles are there? Which colors do they produce?

• Alleles: (5) Cg, Cw, Cr, Cb, Cy
• phenotypes : genotypes
• green-1: Cg Cg
• green-2: Cy Cb
• red: Cr Cr
• white: Cw Cw
• yellow: Cy Cy
• purple: Cr Cb
• orange: Cr Cy
• black: Cr Cg
• blue: Cb Cb

1. Which are dominant and which are recessive?

• Green-1 is dominant to blue, white, yellow, and green-2
• White is recessive to all.
• Green-1 and red show incomplete dominance and produce black.
• Green and blue are codominant.

1. How do the colors combine to produce an overall color?

• CgCg-Green1
• CgCw-Green1
• CgCr-Black
• CgCb-Green1
• CgCy-Green1
• CwCw-White
• CwCr-Red
• CwCb-Blue
• CwCy-Yellow
• CrCr-Red
• CrCb-Purple
• CrCy-Orange
• CbCb-Blue
• CbCy-Green2
• CyCy-Yellow

1. Genotypes of the 4 Starting Organisms:

• Green-1: CgCg
• Green-2: CyCb
• Red: CrCw
• White: CwCw

1. Purple Flowers Genotype

• CbCr

1. Can you construct a pure breeding purple flower from 4 starters?

• No; the only way you could construct a pure breeding purple flower would be by mutation.

1. What can you learn about mutations? Is it easier to mutate from color to white or vice versa? What new colors can you find by mutation?

• It is easier to mutate from color to white than from white to color.

• The new colors found by mutation are:
  • Purple
  • Black
  • Orange
  • Yellow
  • Blue
• These colors are also attainable through cross-breeding other flowers.

1. Make mutant versions of any strain you like. Find one with an unusual color. Find out which alleles are present and if any are new mutant alleles. Define any new mutant alleles you've generated and their dominance/recessivity relationships with the white allele.

• I chose a red mutant flower, and then made it a pure breed.
• It was then crossed with a pure breeding white flower.
• The offspring of the two was all red, thus showing that the red was dominant to the white.

Results

The overall results of the lab showed that based on the genetics of the original set of flowers in Aipotu, there is no way to create a pure-breeding purple flower without some kind of mutation.