J'aime C. Moehlman's Week 4

Activity 1: Part 2

1. Accession number of the chosen sequence: AF089139
2. Subject: 3. The features section of the record talks about subject 3.

FASTA sequences on a word document: Example FASTA Sequences

Activity 1: Part 3

1. Created a workbench account with the user name of jmoehlman.
2. Saved my FASTA data into the Biology Workbench
3. Viewed the Help page for using the ClustalW tool in the nucleic tools.
   • It shows how this tool helps to perform multiple sequence alignment on protein or nucleic sequences.

Activity 2: Part 1

1. added all of the first visit nucleic acid sequences.
2. This data table shows 3 clones from 4 different subjects:
3. Data Table:
   • Subject: Clone:
   • 1 -------- 1,2,3
   • 2 -------- 1,2,3
   • 3 --------- 1,2,3
   • 4 -------- 1,2,3

Unrooted Tree Example

1. On the tree on the link above; it shows all of the clones of each subject clustering together.
2. Subjects 1 and 2 cluster together, while 3 and 4 are separated in different directions.
3. After analyzing the tree it seems that subjects 1 and 2 are similar in their evolutionary relationships. Subjects 3 and 4 vary more than the others because they show much longer branches.

Activity 2: Part 2

1. The subject clones for the first part of this activity were chosen from subject 15.

SUBJECT-----# OF CLONES-----S-----THETA-----MIN DIFF----- MAX DIFF

1. ----------- 13 ----------- 4 ----- 1.74 ----- 0 ------ .2508
2. ----------- 6 ----------- 1 ----- 2.45 ----- 0 ------ 0
3. ----------- 4 ----------- 2 ----- 1.04 ----- 0 ------ 2.499

{J'aime C. Moehlman}