JCA080306-COnstruction of pSB3C6-Bca1025 = pSB3C6-J23045: Difference between revisions
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<tt> | <tt> | ||
[Ptet][StrRBS]-X-[GFP(AGGA)]-[p15A][CmR] | '''[Ptet][StrRBS]-X-[GFP(AGGA)]-[p15A][CmR]'''<br> | ||
N 998 1025R pBca1021-E0040 580bp | N 998 1025R pBca1021-E0040 580bp<br> | ||
O 1025F G00101 pBca1021-E0040 534bp | O 1025F G00101 pBca1021-E0040 534bp<br> | ||
P 998 G00101 N+O 1118bp | P 998 G00101 N+O 1118bp<br> | ||
---- | ---- | ||
P is the overlap PCR of N and O, sub EcoRI/PstI | P is the overlap PCR of N and O, sub EcoRI/PstI<br> | ||
into pAC997 (EcoRI/PstI) | into pAC997 (EcoRI/PstI)<br> | ||
Product is pSB3C6-Bca1025 = J23045 | Product is pSB3C6-Bca1025 = J23045<br> | ||
---- | ---- | ||
ca1025F Forward quickchange for GFP[S56AGGA] | ca1025F Forward quickchange for GFP[S56AGGA]<br> | ||
gaaacagcatgactttttcaagAGGAgccatgcccgaaggttatgtac | gaaacagcatgactttttcaagAGGAgccatgcccgaaggttatgtac<br> | ||
ca1025R Reverse quickchange for GFP[S56AGGA] | ca1025R Reverse quickchange for GFP[S56AGGA]<br> | ||
gtacataaccttcgggcatggcTCCTcttgaaaaagtcatgctgtttc | gtacataaccttcgggcatggcTCCTcttgaaaaagtcatgctgtttc<br> | ||
</tt> | </tt> | ||
Latest revision as of 18:03, 3 August 2006
[Ptet][StrRBS]-X-[GFP(AGGA)]-[p15A][CmR]
N 998 1025R pBca1021-E0040 580bp
O 1025F G00101 pBca1021-E0040 534bp
P 998 G00101 N+O 1118bp
P is the overlap PCR of N and O, sub EcoRI/PstI
into pAC997 (EcoRI/PstI)
Product is pSB3C6-Bca1025 = J23045
ca1025F Forward quickchange for GFP[S56AGGA]
gaaacagcatgactttttcaagAGGAgccatgcccgaaggttatgtac
ca1025R Reverse quickchange for GFP[S56AGGA]
gtacataaccttcgggcatggcTCCTcttgaaaaagtcatgctgtttc
