JCA080306-COnstruction of pSB3C6-Bca1025 = pSB3C6-J23045

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[Ptet][StrRBS]-X-[GFP(AGGA)]-[p15A][CmR] N 998 1025R pBca1021-E0040 580bp O 1025F G00101 pBca1021-E0040 534bp P 998 G00101 N+O 1118bp

P is the overlap PCR of N and O, sub EcoRI/PstI into pAC997 (EcoRI/PstI) Product is pSB3C6-Bca1025 = J23045

ca1025F Forward quickchange for GFP[S56AGGA] gaaacagcatgactttttcaagAGGAgccatgcccgaaggttatgtac ca1025R Reverse quickchange for GFP[S56AGGA] gtacataaccttcgggcatggcTCCTcttgaaaaagtcatgctgtttc

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