JCA080306-COnstruction of pSB3C6-Bca1025 = pSB3C6-J23045

From OpenWetWare

Revision as of 21:03, 3 August 2006 by JCAnderson (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search

[Ptet][StrRBS]-X-[GFP(AGGA)]-[p15A][CmR]
N 998 1025R pBca1021-E0040 580bp
O 1025F G00101 pBca1021-E0040 534bp
P 998 G00101 N+O 1118bp


P is the overlap PCR of N and O, sub EcoRI/PstI
into pAC997 (EcoRI/PstI)
Product is pSB3C6-Bca1025 = J23045


ca1025F Forward quickchange for GFP[S56AGGA]
gaaacagcatgactttttcaagAGGAgccatgcccgaaggttatgtac
ca1025R Reverse quickchange for GFP[S56AGGA]
gtacataaccttcgggcatggcTCCTcttgaaaaagtcatgctgtttc

Personal tools