JCA091306-Construction of lock3l (J23077): Difference between revisions
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==Construction of lock3i (lock3d with 5' extension)== | ==Construction of lock3i (lock3d with 5' extension)== | ||
<tt> | <tt> | ||
PCR ca1028F/ca1001 on | PCR ca1028F/ca1001 on pJ23119-J23049 . . . (865 bp, XbaI/PstI)<br> | ||
Sub into pJ23076 . . . . . . . . . . . . .(SpeI/PstI, large 2105)<br> | Sub into pJ23076 . . . . . . . . . . . . .(SpeI/PstI, large 2105)<br> | ||
Product is pSB3C6-J23077, [Ptet][lock3I][RFP][TT]<br> | Product is pSB3C6-J23077, [Ptet][lock3I][RFP][TT]<br> | ||
<tt> | <tt> | ||
Latest revision as of 11:28, 13 September 2006
Construction of lock3i (lock3d with 5' extension)
PCR ca1028F/ca1001 on pJ23119-J23049 . . . (865 bp, XbaI/PstI)
Sub into pJ23076 . . . . . . . . . . . . .(SpeI/PstI, large 2105)
Product is pSB3C6-J23077, [Ptet][lock3I][RFP][TT]
