JCA091306-Construction of lock3l (J23077)

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Construction of lock3i (lock3d with 5' extension)

PCR ca1028F/ca1001 on pJ23119-J23049 . . . (865 bp, XbaI/PstI)
Sub into pJ23076 . . . . . . . . . . . . .(SpeI/PstI, large 2105)
Product is pSB3C6-J23077, [Ptet][lock3I][RFP][TT]