JCAOligoTutorialPCR

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(New page: left The diagram at left (from fig.cox.miami.edu) is an overview of how PCR works. First of all, what you are ''doing'' is making a mixture of DNAs, ...)
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[[Image:JCATutorial_PCRDiagram.jpg |left]]
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The diagram at left (from fig.cox.miami.edu) is an overview of how PCR works.  First of all, what you are ''doing'' is making a mixture of DNAs, proteins, and chemicals and then putting it through a program of temperature changes.  Inside the mixture is a '''template''' double-stranded DNA, which usually is a small amount of plasmid DNA or genomic DNA, two '''oligonucleotides''', which are short (~20bp) single stranded DNAs, a thermostable DNA polymerase, and the monomer units that make up DNA, referred to as dNTPs.
The diagram at left (from fig.cox.miami.edu) is an overview of how PCR works.  First of all, what you are ''doing'' is making a mixture of DNAs, proteins, and chemicals and then putting it through a program of temperature changes.  Inside the mixture is a '''template''' double-stranded DNA, which usually is a small amount of plasmid DNA or genomic DNA, two '''oligonucleotides''', which are short (~20bp) single stranded DNAs, a thermostable DNA polymerase, and the monomer units that make up DNA, referred to as dNTPs.
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[[Image:JCATutorial_PCRtubes.jpg |right]]
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[[Image:JCATutorial_PCRtubes.jpg |right|200px]]
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[[Image:JCATutorial_PCRthermocycler.jpg |right]]
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[[Image:JCATutorial_PCRthermocycler.jpg |right|200px]]
You make up this sample in little tubes (at right, from www.ga-international.com), and run the temperature program in an instrument called a '''thermocycler''' (at right, from www.uni-saarland.de).
You make up this sample in little tubes (at right, from www.ga-international.com), and run the temperature program in an instrument called a '''thermocycler''' (at right, from www.uni-saarland.de).

Revision as of 16:35, 19 May 2007

The diagram at left (from fig.cox.miami.edu) is an overview of how PCR works. First of all, what you are doing is making a mixture of DNAs, proteins, and chemicals and then putting it through a program of temperature changes. Inside the mixture is a template double-stranded DNA, which usually is a small amount of plasmid DNA or genomic DNA, two oligonucleotides, which are short (~20bp) single stranded DNAs, a thermostable DNA polymerase, and the monomer units that make up DNA, referred to as dNTPs.

You make up this sample in little tubes (at right, from www.ga-international.com), and run the temperature program in an instrument called a thermocycler (at right, from www.uni-saarland.de).

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