JCAOligoTutorialPCR: Difference between revisions
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JCAnderson (talk | contribs) (New page: left The diagram at left (from fig.cox.miami.edu) is an overview of how PCR works. First of all, what you are ''doing'' is making a mixture of DNAs, ...) |
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[[Image:JCATutorial_PCRDiagram.jpg |left]] | [[Image:JCATutorial_PCRDiagram.jpg |left|250px]] | ||
The diagram at left (from fig.cox.miami.edu) is an overview of how PCR works. First of all, what you are ''doing'' is making a mixture of DNAs, proteins, and chemicals and then putting it through a program of temperature changes. Inside the mixture is a '''template''' double-stranded DNA, which usually is a small amount of plasmid DNA or genomic DNA, two '''oligonucleotides''', which are short (~20bp) single stranded DNAs, a thermostable DNA polymerase, and the monomer units that make up DNA, referred to as dNTPs. | The diagram at left (from fig.cox.miami.edu) is an overview of how PCR works. First of all, what you are ''doing'' is making a mixture of DNAs, proteins, and chemicals and then putting it through a program of temperature changes. Inside the mixture is a '''template''' double-stranded DNA, which usually is a small amount of plasmid DNA or genomic DNA, two '''oligonucleotides''', which are short (~20bp) single stranded DNAs, a thermostable DNA polymerase, and the monomer units that make up DNA, referred to as dNTPs. | ||
[[Image:JCATutorial_PCRtubes.jpg |right]] | [[Image:JCATutorial_PCRtubes.jpg |right|200px]] | ||
[[Image:JCATutorial_PCRthermocycler.jpg |right]] | [[Image:JCATutorial_PCRthermocycler.jpg |right|200px]] | ||
You make up this sample in little tubes (at right, from www.ga-international.com), and run the temperature program in an instrument called a '''thermocycler''' (at right, from www.uni-saarland.de). | You make up this sample in little tubes (at right, from www.ga-international.com), and run the temperature program in an instrument called a '''thermocycler''' (at right, from www.uni-saarland.de). | ||
Revision as of 13:35, 19 May 2007
The diagram at left (from fig.cox.miami.edu) is an overview of how PCR works. First of all, what you are doing is making a mixture of DNAs, proteins, and chemicals and then putting it through a program of temperature changes. Inside the mixture is a template double-stranded DNA, which usually is a small amount of plasmid DNA or genomic DNA, two oligonucleotides, which are short (~20bp) single stranded DNAs, a thermostable DNA polymerase, and the monomer units that make up DNA, referred to as dNTPs.
You make up this sample in little tubes (at right, from www.ga-international.com), and run the temperature program in an instrument called a thermocycler (at right, from www.uni-saarland.de).
