JL071106-Knockout of oriTF/traM of pOX38

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PCR FORlamF/JL7 on pKD3 (991 bp, DpnI)
Transform pOX38/pKD46
Product is J23013

JL7 Reverse knockout oligo for oriT/traM
cataaaaactctgatatttgaacgaagtcaaatttcgtttATGGGAATTAGCCATGGTCC

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