Jacobs:Back Door: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Line 28: | Line 28: | ||
*[[Jacobs:Protocol Loading Mice|Loading Mice]] | *[[Jacobs:Protocol Loading Mice|Loading Mice]] | ||
*[[Jacobs:Protocol OFF (Large Chambers)|OFF (Large Chambers)]] | *[[Jacobs:Protocol OFF (Large Chambers)|OFF (Large Chambers)]] | ||
===Teaching Protocols=== | ===Teaching Protocols=== |
Revision as of 10:20, 9 September 2009
Useful Links
Equipment and Schedules
Protocols
Research Protocols
- Safety
- Freezing and Thawing Cells
- Actin Staining
- Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel
- Protein Gel Staining Using Coomassie Blue Protein Stain
- Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)and Coomassie Staining of Polyacrylamide Gel for Protein
- Agarose Formaldehyde Gel Preparation for RNA Electrophoresis
- Total Protein Isolation Using RIPA Lysis Buffer
- Transfecting NIH 3T3s with GFP-Actin
- 10 bp DNA Ladder
- Collagen Gel Contraction 2
- Agarose Formaldehyde Gel Preparation for RNA and Electrophoresis
- Loading Mice
- OFF (Large Chambers)