Jacobs:Back Door: Difference between revisions
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*[[Jacobs:Protocol Flow chamber calibration|Flow chamber calibration]] | *[[Jacobs:Protocol Flow chamber calibration|Flow chamber calibration]] | ||
*[[Jacobs:Protocol siRNA transfection|siRNA transfection]] | *[[Jacobs:Protocol siRNA transfection|siRNA transfection]] | ||
*[[Jacobs:Protocol Separating and Tagging of Mice|Separating and Tagging of Mice | *[[Jacobs:Protocol Separating and Tagging of Mice|Separating and Tagging of Mice]] | ||
===Teaching Protocols=== | ===Teaching Protocols=== | ||
Revision as of 09:43, 30 April 2010
Useful Links / Meetings
Equipment and Schedules
Protocols
Research Protocols
- Safety
- Freezing and Thawing Cells
- Actin Staining
- Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel
- Protein Gel Staining Using Coomassie Blue Protein Stain
- Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)and Coomassie Staining of Polyacrylamide Gel for Protein
- Agarose Formaldehyde Gel Preparation for RNA Electrophoresis
- Total Protein Isolation Using RIPA Lysis Buffer
- Transfecting NIH 3T3s with GFP-Actin
- 10 bp DNA Ladder
- Collagen Gel Contraction 2
- Agarose Formaldehyde Gel Preparation for RNA and Electrophoresis
- Loading Mice
- OFF (Large Chambers)
- Flow chamber calibration
- siRNA transfection
- Separating and Tagging of Mice
