Jacobs:Back Door
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*[[Jacobs:Cell Culture Numbers| Cell Culture numbers]] | *[[Jacobs:Cell Culture Numbers| Cell Culture numbers]] | ||
*[[Jacobs: Troubleshooting Cell Culture| Troubleshooting Cell Culture]] | *[[Jacobs: Troubleshooting Cell Culture| Troubleshooting Cell Culture]] | ||
| - | *[[Jacobs: | + | *[[Jacobs: Leica MP Microscope Protocol| Lecia MP Microscope Protocol]] |
| + | *[[Jacobs: In vivo microCT scanning protocol for live mice| In vivo microCT scanning protocol for live mice]] | ||
===Teaching Protocols=== | ===Teaching Protocols=== | ||
Revision as of 12:28, 27 January 2011
Contents |
Useful Links / Meetings
Equipment and Schedules
Protocols
Research Protocols
- Safety
- Freezing and Thawing Cells
- Actin Staining
- Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel
- Protein Gel Staining Using Coomassie Blue Protein Stain
- Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)and Coomassie Staining of Polyacrylamide Gel for Protein
- Agarose Formaldehyde Gel Preparation for RNA Electrophoresis
- Total Protein Isolation Using RIPA Lysis Buffer
- Transfecting NIH 3T3s with GFP-Actin
- 10 bp DNA Ladder
- Collagen Gel Contraction 2
- Agarose Formaldehyde Gel Preparation for RNA and Electrophoresis
- Loading Mice
- OFF (Large Chambers)
- Flow chamber calibration
- siRNA transfection
- Separating and Tagging of Mice
- Genotyping Tailsnips
- Tubulin Immunostaining Protocol
- Confocal Imaging of Flow
- Cell Culture numbers
- Troubleshooting Cell Culture
- Lecia MP Microscope Protocol
- In vivo microCT scanning protocol for live mice
