Jacobs:Back Door: Difference between revisions

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(New page: {{Template:Jacobs}} ==Useful Links== *[http://parts.mit.edu/igem07/index.php/Podcasts Believe] in Synthetic Biology ==Equipment and Schedules== *Wish List *[[Jacobs...)
 
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==Useful Links==
==Useful Links / Meetings==
*[http://parts.mit.edu/igem07/index.php/Podcasts Believe] in Synthetic Biology
*[https://suehsaps3.stanford.edu/stanford/ Chemtracker]
*[http://www.ors.org/ ORS]
*[http://www.asmeconferences.org/SBC2012/ ASME SBC]
*[http://www.asbmr.org/ ASBMR]
*[http://www.bmes.org/ BMES]


==Equipment and Schedules==
==Protocols==
*[[Jacobs:Wish_list|Wish List]]
===Research Protocols===
*[[Jacobs:TC Hood Schedule|TC Hood Schedule]]
*[[Jacobs:Protocol Safety|Safety]]
*[[Jacobs:Meeting Schedule|Lab Meeting Schedule]]
*[[Jacobs:Protocol Freezing and Thawing Cells|Freezing and Thawing Cells]]
*[[Jacobs:Lab Jobs|Lab Jobs]]
*[[Jacobs:Protocol Actin Staining|Actin Staining]]
*[[Jacobs:Freezer Defrost Schedule|Freezer Defrost Schedule]]
*[[Jacobs:Protocol Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel|Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel]]
*[[Jacobs:Protocol Protein Gel Staining Using Coomassie Blue Protein Stain|Protein Gel Staining Using Coomassie Blue Protein Stain]]
*[[Jacobs:Protocol Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Coomassie Staining of Polyacrylamide Gel for Protein|Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)and Coomassie Staining of Polyacrylamide Gel for Protein]]
*[[Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA Electrophoresis|Agarose Formaldehyde Gel Preparation for RNA Electrophoresis]]
*[[Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer|Total Protein Isolation Using RIPA Lysis Buffer]]
*[[Jacobs:Protocol Transfecting NIH 3T3s with GFP-Actin|Transfecting NIH 3T3s with GFP-Actin]]
*[[Jacobs:Protocol 10 bp DNA Ladder|10 bp DNA Ladder]]
*[[Jacobs:Protocol Collagen Gel Contraction 2|Collagen Gel Contraction 2]]
*[[Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA and Electrophoresis|Agarose Formaldehyde Gel Preparation for RNA and Electrophoresis]]
*[[Jacobs:Protocol Loading Mice|Loading Mice]]
*[[Jacobs:Protocol OFF (Large Chambers)|OFF (Large Chambers)]]
*[[Jacobs:Protocol Flow chamber calibration|Flow chamber calibration]]
*[[Jacobs:Protocol siRNA transfection|siRNA transfection]]
*[[Jacobs:Protocol Separating and Tagging of Mice|Separating and Tagging of Mice]]
*[[Jacobs:Genotyping Tailsnips|Genotyping Tailsnips]]
*[[Jacobs:Tubulin Immunostaining Protocol|Tubulin Immunostaining Protocol]]
*[[Jacobs:Confocal Imaging of Flow| Confocal Imaging of Flow]]
*[[Jacobs:Cell Culture Numbers| Cell Culture numbers]]
*[[Jacobs: Troubleshooting Cell Culture| Troubleshooting Cell Culture]]
*[[Jacobs: Leica MP Microscope Protocol| Lecia MP Microscope Protocol]]
*[[Jacobs: Protocol In vivo microCT scanning protocol for live mice| In vivo microCT scanning protocol for live mice]]
*[[Jacobs: Isolation of Primary Bone Cells| Isolation of Primary Bone Cells]]
*[[Jacobs: Cell Culture of MLOY-4 Osteocyte Cells]]
*[[Jacobs: Cell Culture of IMCD Kidney cells]]
*[[Jacobs:Protocol Cell Culture of MDCK cells | Cell Culture of MDCK cells]]
*[[Jacobs:Protocol Autoclave | Autoclave]]
*[[Jacobs:Protocol Alkaline Phosphatase Assay | Alkaline Phosphatase Assay]]
*[[Jacobs:Protocol Neon Transfection | Neon Transfection]]
*[[Jacobs:Protocol Coating Slides with Fibronectin | Coating Slides with Fibronectin]]
*[[Jacobs:Protocol Osteocyte Markers|Osteocyte Markers]]
*[[Jacobs:Protocol Printers | Printers]]
*[[Jacobs: Protocol Immunostaining for TRPV4 and primary cilia | Immunostaining for TRPV4 and PC]]
*[[Jacobs: Protocol Preparation of primary osteocytes and MDCK cells for immunostaining | Preparation of primary osteocytes and MDCK cells for immunostaining]]
 
===Teaching Protocols===
*[[Jacobs:Protocol BCA_Total_Protein|BCA Total Protein]]
 
*[[Jacobs:Protocol Adipocyte Differentiation|Adipocyte Differentiation]]
 
*[[Jacobs:Protocol Oil Red O Staining|Oil Red O Staining]]
 
*[[Jacobs:Protocol RNA Agarose Gel|RNA Agarose Gel]]
 
*[[Jacobs:Protocol Collagen Gel Contraction|Collagen Gel Contraction]]
 
*[[Jacobs:Protocol Real-Time PCR|Real-Time PCR]]
 
*[[Jacobs:Protocol Pipetting Exercise|Pipetting Exercise]]
 
*[[Jacobs:Protocol Total Protein Isolation using RIPA buffer|Total Protein Isolation using RIPA buffer]]
 
*[[Jacobs:Protocol Total Protein Isolation using RIPA buffer 2|Total Protein Isolation using RIPA buffer 2]]
 
*[[Jacobs:Protocol RNA Sample Loading Buffer|RNA Sample Loading Buffer]]
 
*[[Jacobs:Protocol Total RNA Isolation using Tri Reagent|Total RNA Isolation using Tri Reagent]]
 
*[[Jacobs:Protocol Subculturing 3T3 Fibroblasts|Subculturing 3T3 Fibroblasts]]
 
*[[Jacobs:Protocol NuPAGE Electrophoresis and Western Blotting for Proteins|NuPAGE Electrophoresis and Western Blotting for Proteins]]
 
==Miscellaneous==


==Protocols==
* [[Photos:Jacobs photos]]
*[[Jacobs:Protocol BCA_Total_Protein]]
* [[Jacobs:Library|Library]]
* [http://www.stanford.edu/group/cmbl/movies.htm  Movies]
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Revision as of 18:18, 3 December 2011



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Useful Links / Meetings

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Research Protocols

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