Jacobs:Back Door: Difference between revisions
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==Useful Links / Meetings== | ==Useful Links / Meetings== | ||
*[https://suehsaps3.stanford.edu/stanford/ Chemtracker] | *[https://suehsaps3.stanford.edu/stanford/ Chemtracker] | ||
*[http://www.ors.org/ ORS] | *[http://www.ors.org/ ORS] | ||
*[http://asmeconferences.org/ | *[http://www.asmeconferences.org/SBC2012/ ASME SBC] | ||
*[http://www.asbmr.org/ ASBMR] | *[http://www.asbmr.org/ ASBMR] | ||
*[http://www.bmes.org/ BMES] | *[http://www.bmes.org/ BMES] | ||
==Protocols== | ==Protocols== | ||
| Line 33: | Line 27: | ||
*[[Jacobs:Protocol OFF (Large Chambers)|OFF (Large Chambers)]] | *[[Jacobs:Protocol OFF (Large Chambers)|OFF (Large Chambers)]] | ||
*[[Jacobs:Protocol Flow chamber calibration|Flow chamber calibration]] | *[[Jacobs:Protocol Flow chamber calibration|Flow chamber calibration]] | ||
*[[Jacobs:Protocol siRNA transfection|siRNA transfection]] | |||
*[[Jacobs:Protocol Separating and Tagging of Mice|Separating and Tagging of Mice]] | |||
*[[Jacobs:Genotyping Tailsnips|Genotyping Tailsnips]] | |||
*[[Jacobs:Tubulin Immunostaining Protocol|Tubulin Immunostaining Protocol]] | |||
*[[Jacobs:Confocal Imaging of Flow| Confocal Imaging of Flow]] | |||
*[[Jacobs:Cell Culture Numbers| Cell Culture numbers]] | |||
*[[Jacobs: Troubleshooting Cell Culture| Troubleshooting Cell Culture]] | |||
*[[Jacobs: Leica MP Microscope Protocol| Lecia MP Microscope Protocol]] | |||
*[[Jacobs: Protocol In vivo microCT scanning protocol for live mice| In vivo microCT scanning protocol for live mice]] | |||
*[[Jacobs: Isolation of Primary Bone Cells| Isolation of Primary Bone Cells]] | |||
*[[Jacobs: Cell Culture of MLOY-4 Osteocyte Cells]] | |||
*[[Jacobs: Cell Culture of IMCD Kidney cells]] | |||
*[[Jacobs:Protocol Cell Culture of MDCK cells | Cell Culture of MDCK cells]] | |||
*[[Jacobs:Protocol Autoclave | Autoclave]] | |||
*[[Jacobs:Protocol Alkaline Phosphatase Assay | Alkaline Phosphatase Assay]] | |||
*[[Jacobs:Protocol Neon Transfection | Neon Transfection]] | |||
*[[Jacobs:Protocol Coating Slides with Fibronectin | Coating Slides with Fibronectin]] | |||
*[[Jacobs:Protocol Osteocyte Markers|Osteocyte Markers]] | |||
*[[Jacobs:Protocol Printers | Printers]] | |||
*[[Jacobs: Protocol Immunostaining for TRPV4 and primary cilia | Immunostaining for TRPV4 and PC]] | |||
*[[Jacobs: Protocol Preparation of primary osteocytes and MDCK cells for immunostaining | Preparation of primary osteocytes and MDCK cells for immunostaining]] | |||
===Teaching Protocols=== | ===Teaching Protocols=== | ||
| Line 63: | Line 78: | ||
==Miscellaneous== | ==Miscellaneous== | ||
* [ | * [[Photos:Jacobs photos]] | ||
* [[Jacobs:Library|Library]] | |||
* [http://www.stanford.edu/group/cmbl/movies.htm Movies] | * [http://www.stanford.edu/group/cmbl/movies.htm Movies] | ||
</div> | |||
Revision as of 18:18, 3 December 2011
Useful Links / Meetings
Protocols
Research Protocols
- Safety
- Freezing and Thawing Cells
- Actin Staining
- Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel
- Protein Gel Staining Using Coomassie Blue Protein Stain
- Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)and Coomassie Staining of Polyacrylamide Gel for Protein
- Agarose Formaldehyde Gel Preparation for RNA Electrophoresis
- Total Protein Isolation Using RIPA Lysis Buffer
- Transfecting NIH 3T3s with GFP-Actin
- 10 bp DNA Ladder
- Collagen Gel Contraction 2
- Agarose Formaldehyde Gel Preparation for RNA and Electrophoresis
- Loading Mice
- OFF (Large Chambers)
- Flow chamber calibration
- siRNA transfection
- Separating and Tagging of Mice
- Genotyping Tailsnips
- Tubulin Immunostaining Protocol
- Confocal Imaging of Flow
- Cell Culture numbers
- Troubleshooting Cell Culture
- Lecia MP Microscope Protocol
- In vivo microCT scanning protocol for live mice
- Isolation of Primary Bone Cells
- Jacobs: Cell Culture of MLOY-4 Osteocyte Cells
- Jacobs: Cell Culture of IMCD Kidney cells
- Cell Culture of MDCK cells
- Autoclave
- Alkaline Phosphatase Assay
- Neon Transfection
- Coating Slides with Fibronectin
- Osteocyte Markers
- Printers
- Immunostaining for TRPV4 and PC
- Preparation of primary osteocytes and MDCK cells for immunostaining
