Jacobs:Back Door: Difference between revisions

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{{Template:Jacobs}}
{{Template:Jacobs}}


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==Useful Links / Meetings==
==Useful Links / Meetings==
*[https://suehsaps3.stanford.edu/stanford/ Chemtracker]
*[https://suehsaps3.stanford.edu/stanford/ Chemtracker]
*[http://www.ors.org/ ORS]
*[http://www.ors.org/ ORS]
*[http://asmeconferences.org/sbc2010/ ASME SBC]
*[http://www.asmeconferences.org/SBC2012/ ASME SBC]
*[http://www.asbmr.org/ ASBMR]
*[http://www.asbmr.org/ ASBMR]
*[http://www.bmes.org/ BMES]
*[http://www.bmes.org/ BMES]
==Equipment and Schedules==
*[[Jacobs:Wish_list|Wish List]]
*[[Jacobs:TC Hood Schedule|TC Hood Schedule]]
*[[Jacobs:Meeting Schedule|Lab Meeting Schedule]]
*[[Jacobs:Lab Jobs|Lab Jobs]]
*[[Jacobs:Freezer Defrost Schedule|Freezer Defrost Schedule]]


==Protocols==
==Protocols==
===Research Protocols===
===Research Protocols===
*[[Jacobs:Protocol Safety|Safety]]
*General
*[[Jacobs:Protocol Freezing and Thawing Cells|Freezing and Thawing Cells]]
**[[Jacobs:Protocol Printers | Printers]]
*[[Jacobs:Protocol Actin Staining|Actin Staining]]
**[[Jacobs:Protocol Safety|Safety]]
*[[Jacobs:Protocol Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel|Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel]]
**[[Jacobs:Protocol Autoclave | Autoclave]]
*[[Jacobs:Protocol Protein Gel Staining Using Coomassie Blue Protein Stain|Protein Gel Staining Using Coomassie Blue Protein Stain]]
**[[Jacobs: Leica MP Microscope Protocol| Leica MP Microscope]]
*[[Jacobs:Protocol Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Coomassie Staining of Polyacrylamide Gel for Protein|Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)and Coomassie Staining of Polyacrylamide Gel for Protein]]
*Cells
*[[Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA Electrophoresis|Agarose Formaldehyde Gel Preparation for RNA Electrophoresis]]
**Culture
*[[Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer|Total Protein Isolation Using RIPA Lysis Buffer]]
***[[Jacobs: Cell Culture of MLOY-4 Osteocyte Cells | Culture of MLO-Y4s]]
*[[Jacobs:Protocol Transfecting NIH 3T3s with GFP-Actin|Transfecting NIH 3T3s with GFP-Actin]]
***[[Jacobs: Cell Culture of IMCD Kidney cells | Culture of IMCDs]]
*[[Jacobs:Protocol 10 bp DNA Ladder|10 bp DNA Ladder]]
***[[Jacobs:Protocol Cell Culture of MDCK cells |Culture of MDCKs]]
*[[Jacobs:Protocol Collagen Gel Contraction 2|Collagen Gel Contraction 2]]
***[[Jacobs:Protocol Subculturing 3T3 Fibroblasts|Culture of 3T3 Fibroblasts]]
*[[Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA and Electrophoresis|Agarose Formaldehyde Gel Preparation for RNA and Electrophoresis]]
***[[Jacobs:Cell Culture Numbers| Cell Culture numbers]]
*[[Jacobs:Protocol Loading Mice|Loading Mice]]
***[[Jacobs: Troubleshooting Cell Culture| Troubleshooting Cell Culture]]
*[[Jacobs:Protocol OFF (Large Chambers)|OFF (Large Chambers)]]
***[[Jacobs:Protocol Freezing and Thawing Cells|Freezing and Thawing Cells]]
*[[Jacobs:Protocol Flow chamber calibration|Flow chamber calibration]]
**Transfection
*[[Jacobs:Protocol siRNA transfection|siRNA transfection]]
***[[Jacobs:Protocol siRNA transfection|siRNA transfection]]
*[[Jacobs:Protocol Separating and Tagging of Mice|Separating and Tagging of Mice]]
***[[Jacobs:Protocol Neon Transfection | Neon Transfection]]
*[[Jacobs:Genotyping Tailsnips|Genotyping Tailsnips]]
***[[Jacobs:Protocol Transfecting NIH 3T3s with GFP-Actin|Transfecting NIH 3T3s with GFP-Actin]]
*[[Jacobs:Tubulin Immunostaining Protocol|Tubulin Immunostaining Protocol]]
**[[Jacobs:Protocol Coating Slides with Fibronectin | Coating with Fibronectin]]
*[[Jacobs:Confocal Imaging of Flow| Confocal Imaging of Flow]]
**[[Jacobs: Isolation of Primary Bone Cells| Isolation of Primary Bone Cells]]
*[[Jacobs:Cell Culture Numbers| Cell Culture numbers]]
**[[Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer|Total Protein Isolation Using RIPA Lysis Buffer]]
*[[Jacobs: Troubleshooting Cell Culture| Troubleshooting Cell Culture]]
**[[Jacobs:Protocol Total RNA Isolation using Tri Reagent|Total RNA Isolation using Tri Reagent]]
*[[Jacobs: Leica MP Microscope Protocol| Lecia MP Microscope Protocol]]
*Flow
*[[Jacobs: Protocol In vivo microCT scanning protocol for live mice| In vivo microCT scanning protocol for live mice]]
**[[Jacobs:Protocol OFF (Large Chambers)|OFF (Large Chambers)]]
*[[Jacobs: Isolation of Primary Bone Cells| Isolation of Primary Bone Cells]]
**[[Jacobs:Protocol Flow chamber calibration|Flow chamber calibration]]
*[[Jacobs: Cell Culture of MLOY-4 Osteocyte Cells]]
**[[Jacobs:Confocal Imaging of Flow| Confocal imaging of primary cilia under flow]]
*[[Jacobs: Cell Culture of IMCD Kidney cells]]
 
*[[Jacobs:Protocol Autoclave | Autoclave]]
*Immunocytochemistry
*[[Jacobs:Protocol Alkaline Phosphatase Assay | Alkaline Phosphatase Assay]]
**[[Jacobs: Protocol Immunostaining for TRPV4 and primary cilia | Immunostaining for TRPV4 and PC]]
*[[Jacobs:Protocol Neon Transfection | Neon Transfection]]
**[[Jacobs: Protocol Preparation of primary osteocytes and MDCK cells for immunostaining | Preparation of primary osteocytes and MDCK cells for immunostaining]]
*[[Jacobs:Protocol Coating Slides with Fibronectin | Coating Slides with Fibronectin]]
**[[Jacobs:Protocol Actin Staining|Actin Staining]]
*[[Jacobs:Protocol Osteocyte Markers|Osteocyte Markers]]
**[[Jacobs:Tubulin Immunostaining Protocol|Tubulin Immunostaining]]
*[[Jacobs:Protocol Printers | Printers]]
*Assays
*[[Jacobs: Protocol Immunostaining for TRPV4 and primary cilia | Immunostaining for TRPV4 and PC]]
**[[Jacobs:Protocol BCA_Total_Protein|BCA Total Protein Content]]
*[[Jacobs: Protocol Preparation of primary osteocytes and MDCK cells for immunostaining | Preparation of primary osteocytes and MDCK cells for immunostaining]]
**[[Jacobs:Protocol Real-Time PCR|Real-Time RT qPCR]]
**[[Jacobs:Protocol Osteocyte Markers|Gene expression of osteocyte markers]]
**[[Jacobs:Protocol Alkaline Phosphatase Assay | Alkaline Phosphatase]]
*Gels & blots
**Protein
***[[Jacobs:Protocol NuPAGE Electrophoresis and Western Blotting for Proteins|NuPAGE Electrophoresis and Western Blotting for Proteins]]
***[[Jacobs:Protocol Protein Gel Staining Using Coomassie Blue Protein Stain|Protein Gel Staining Using Coomassie Blue Protein Stain]]
***[[Jacobs:Protocol Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Coomassie Staining of Polyacrylamide Gel for Protein|Protein SDS-PAGE and Coomassie Staining of Polyacrylamide Gel for Protein]]
**DNA & RNA
***[[Jacobs:Protocol Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel|Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel]]
***[[Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA and Electrophoresis|Agarose Formaldehyde Gel Preparation for RNA Electrophoresis (1)]]
***[[Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA Electrophoresis|Agarose Formaldehyde Gel Preparation for RNA Electrophoresis (2)]]
***[[Jacobs:Protocol 10 bp DNA Ladder|10 bp DNA Ladder]]
* Mice
**[[Jacobs:Protocol Separating and Tagging of Mice|Separating and Tagging of Mice]]
**[[Jacobs:Genotyping Tailsnips|Genotyping by qPCR]]
**[[Media:Genotyping Tailsnips.doc | Genotyping by agarose gel]]
**[[Jacobs:Protocol Loading Mice|Loading Mice]]
**[[Jacobs: Protocol In vivo microCT scanning protocol for live mice| In vivo microCT scanning protocol for live mice]]


===Teaching Protocols===
===Teaching Protocols===
*[[Jacobs:Protocol BCA_Total_Protein|BCA Total Protein]]
*[[Jacobs:Protocol Pipetting Exercise|Pipetting Exercise]]
 
*[[Jacobs:Protocol Adipocyte Differentiation|Adipocyte Differentiation]]
*[[Jacobs:Protocol Adipocyte Differentiation|Adipocyte Differentiation]]
*[[Jacobs:Protocol Oil Red O Staining|Oil Red O Staining]]
*[[Jacobs:Protocol Oil Red O Staining|Oil Red O Staining]]
*[[Jacobs:Protocol RNA Agarose Gel|RNA Agarose Gel]]
*[[Jacobs:Protocol RNA Agarose Gel|RNA Agarose Gel]]
*[[Jacobs:Protocol Collagen Gel Contraction|Collagen Gel Contraction]]
*[[Jacobs:Protocol Collagen Gel Contraction|Collagen Gel Contraction]]
 
*[[Jacobs:Protocol Collagen Gel Contraction 2|Collagen Gel Contraction 2]]
*[[Jacobs:Protocol Real-Time PCR|Real-Time PCR]]
 
*[[Jacobs:Protocol Pipetting Exercise|Pipetting Exercise]]
 
*[[Jacobs:Protocol Total Protein Isolation using RIPA buffer|Total Protein Isolation using RIPA buffer]]
*[[Jacobs:Protocol Total Protein Isolation using RIPA buffer|Total Protein Isolation using RIPA buffer]]
*[[Jacobs:Protocol Total Protein Isolation using RIPA buffer 2|Total Protein Isolation using RIPA buffer 2]]
*[[Jacobs:Protocol Total Protein Isolation using RIPA buffer 2|Total Protein Isolation using RIPA buffer 2]]
*[[Jacobs:Protocol RNA Sample Loading Buffer|RNA Sample Loading Buffer]]
*[[Jacobs:Protocol RNA Sample Loading Buffer|RNA Sample Loading Buffer]]
*[[Jacobs:Protocol Total RNA Isolation using Tri Reagent|Total RNA Isolation using Tri Reagent]]
*[[Jacobs:Protocol Subculturing 3T3 Fibroblasts|Subculturing 3T3 Fibroblasts]]
*[[Jacobs:Protocol NuPAGE Electrophoresis and Western Blotting for Proteins|NuPAGE Electrophoresis and Western Blotting for Proteins]]


==Miscellaneous==
==Miscellaneous==


* [[Photos:Jacobs photos]]
* [[Photos:Jacobs photos]]
* [[Jacobs:Library|Library]]
* [http://www.stanford.edu/group/cmbl/movies.htm  Movies]
* [http://www.stanford.edu/group/cmbl/movies.htm  Movies]
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Latest revision as of 12:49, 2 December 2014



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