Jacobs:Back Door: Difference between revisions
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*[[Jacobs:Protocol RNA Sample Loading Buffer|RNA Sample Loading Buffer]] | *[[Jacobs:Protocol RNA Sample Loading Buffer|RNA Sample Loading Buffer]] | ||
*[[Jacobs:Protocol Total RNA Isolation using Tri Reagent|Total RNA Isolation using Tri Reagent]] | *[[Jacobs:Protocol Total RNA Isolation using Tri Reagent|Total RNA Isolation using Tri Reagent]] | ||
*[[Jacobs:Protocol Subculturing 3T3 Fibroblasts|Subculturing 3T3 Fibroblasts]] | |||
*[[Jacobs:Protocol NuPAGE Electrophoresis and Western Blotting for Proteins|NuPAGE Electrophoresis and Western Blotting for Proteins]] | |||
*[[Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA and Electrophoresis|Agarose Formaldehyde Gel Preparation for RNA and Electrophoresis]] | |||
===Teaching Protocols=== | ===Teaching Protocols=== | ||
Revision as of 10:11, 17 June 2008
Useful Links
Equipment and Schedules
Protocols
Research Protocols
- Safety
- Actin Staining
- Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel
- Protein Gel Staining Using Coomassie Blue Protein Stain
- Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)and Coomassie Staining of Polyacrylamide Gel for Protein
- Agarose Formaldehyde Gel Preparation for RNA Electrophoresis
- Total Protein Isolation Using RIPA Lysis Buffer
- Transfecting NIH 3T3s with GFP-Actin
- 10 bp DNA Ladder
- Collagen Gel Contraction 2
- Total Protein Isolation using RIPA buffer
- Total Protein Isolation using RIPA buffer 2
- RNA Sample Loading Buffer
- Total RNA Isolation using Tri Reagent
- Subculturing 3T3 Fibroblasts
