Jacobs:Back Door: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
David Hoey (talk | contribs) |
David Hoey (talk | contribs) |
||
Line 39: | Line 39: | ||
*[[Jacobs:Confocal Imaging of Flow| Confocal Imaging of Flow]] | *[[Jacobs:Confocal Imaging of Flow| Confocal Imaging of Flow]] | ||
*[[Jacobs:Cell Culture Numbers| Cell Culture numbers]] | *[[Jacobs:Cell Culture Numbers| Cell Culture numbers]] | ||
*[[Jacobs: Troubleshooting Cell Culture| Troubleshooting Cell Culture]] | |||
===Teaching Protocols=== | ===Teaching Protocols=== |
Revision as of 07:25, 10 May 2010
Useful Links / Meetings
Equipment and Schedules
Protocols
Research Protocols
- Safety
- Freezing and Thawing Cells
- Actin Staining
- Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel
- Protein Gel Staining Using Coomassie Blue Protein Stain
- Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)and Coomassie Staining of Polyacrylamide Gel for Protein
- Agarose Formaldehyde Gel Preparation for RNA Electrophoresis
- Total Protein Isolation Using RIPA Lysis Buffer
- Transfecting NIH 3T3s with GFP-Actin
- 10 bp DNA Ladder
- Collagen Gel Contraction 2
- Agarose Formaldehyde Gel Preparation for RNA and Electrophoresis
- Loading Mice
- OFF (Large Chambers)
- Flow chamber calibration
- siRNA transfection
- Separating and Tagging of Mice
- Genotyping Tailsnips
- Tubulin Immunostaining Protocol
- Confocal Imaging of Flow
- Cell Culture numbers
- Troubleshooting Cell Culture