Jacobs:Back Door

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Useful Links / Meetings

• Chemtracker
• ORS
• ASME SBC
• ASBMR
• BMES

Equipment and Schedules

• Wish List
• TC Hood Schedule
• Lab Meeting Schedule
• Lab Jobs
• Freezer Defrost Schedule

Protocols

Research Protocols

• Safety
• Freezing and Thawing Cells
• Actin Staining
• Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel
• Protein Gel Staining Using Coomassie Blue Protein Stain
• Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)and Coomassie Staining of Polyacrylamide Gel for Protein
• Agarose Formaldehyde Gel Preparation for RNA Electrophoresis
• Total Protein Isolation Using RIPA Lysis Buffer
• Transfecting NIH 3T3s with GFP-Actin
• 10 bp DNA Ladder
• Collagen Gel Contraction 2
• Agarose Formaldehyde Gel Preparation for RNA and Electrophoresis
• Loading Mice
• OFF (Large Chambers)
• Flow chamber calibration
• siRNA transfection
• Separating and Tagging of Mice
• Genotyping Tailsnips
• Tubulin Immunostaining Protocol
• Confocal Imaging of Flow
• Cell Culture numbers
• Troubleshooting Cell Culture
• Lecia MP Microscope Protocol
• In vivo microCT scanning protocol for live mice
• Isolation of Primary Bone Cells
• Jacobs: Cell Culture of MLOY-4 Osteocyte Cells
• Jacobs: Cell Culture of IMCD Kidney cells
• Cell Culture of MDCK cells
• Autoclave
• Alkaline Phosphatase Assay
• Neon Transfection
• Coating Slides with Fibronectin
• Osteocyte Markers
• Printers
• Immunostaining for TRPV4 and PC
• Preparation of primary osteocytes and MDCK cells for immunostaining

Teaching Protocols

• BCA Total Protein

• Adipocyte Differentiation

• Oil Red O Staining

• RNA Agarose Gel

• Collagen Gel Contraction

• Real-Time PCR

• Pipetting Exercise

• Total Protein Isolation using RIPA buffer

• Total Protein Isolation using RIPA buffer 2

• RNA Sample Loading Buffer

• Total RNA Isolation using Tri Reagent

• Subculturing 3T3 Fibroblasts

• NuPAGE Electrophoresis and Western Blotting for Proteins

Miscellaneous

• Photos:Jacobs photos
• Movies