Jacobs:Library

Transfection

• pEGFP-N1 Information
• Enhancer dependent expression of human k immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation- Potter, Weir, Leder
• Transfection Slides
• Electroporation for the efficient transfection of mammalian cells with DNA – Chu, Hayakawa, Berg

Stem Cells

• Sonic hedgehog signaling is decoded by calcium spike activity in the developing spinal cord – Belgacem Borodinsky
• Induced pluripotent stem cells generated without viral integration – Stadtfeld
• Induced pluripotent stem cell lines derived from Human somatic cells – Yu
• Human embryonic stem cells in culture poses primary cilia with hedgehog signaling machinery – Kiprilov, Hirsch
• Generation of human induced pluripotent stem cells from dermal fibroblasts – Lowry, Plath
• Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors – Takahashi, Yamanaka
• Generation of germline-competent induced pluripotent stem cells – Okita, Yamanaka

Mat’l Info

• Info on SSTR-3 antibody

MLO-Y4

• Establishment of an Osteocyte-like Cell Line, MLO-Y4- Kato, Bonewald

Imaging

• Analyzing primary cilia by multiphoton microscopy- Farnum Donnelly
• Polarized florescence microscopy of individual and many kinesin motors bound to axonemal microtubules – Peterman, Moerner
• The Primary Cilium of connective Tissue Cells: Imaging by Multiphoton Microscopy – Donnelly, Farnum
• Planar polarization of node cells determines the rotational axis of node cilia – Hashimoto, Hamada
• Live cell imaging of cytoskeletal dynamics in neurons using fluorescence photoactivation – Gauthier, Brandt
• Super-Resolution Dynamic Imaging of dendritic spines using a low affinity photoconvertible actin probe – Izeddin, Dahan

Misc

• Ciliopathies – Hildebrandt, Katsanis
• DISC1 regulates primary cilia that display specific dopamine receptors – Marley, Zastrow
• Insufficiency of BUBR1, a mitotic spindle checkpoint regulator, causes impaired ciliogenesis in vertebrates – Miyamoto, Matsuura
• Flexural rigidity of Echinoderm sperm flagella – Ishijima, Hiramoto
• Restricted expression of somotostatin receptor 3 to primary cilia in the pancreatic islets and adenohypophysis of mice – Iwanaga, Takahashi- Iwanaga
• Isolation of primary cilia for morphological analysis – Huang, LaRusso
• Beyond 9+0: noncanonical axoneme structures characterize sensory cilia from protists to humans – Gluenz, Gull
• In vitro effects of taxol on ciliogenesis in quail oviduct – Bouvieux-Ulrich, Sandoz
• Primary cilia in vertebrate corneal endothelial cells - Collin, Collin
• Rabs and other small GTPases in ciliary transport – Lim, Tang
• Primary cilia control telencephalic patterning and morphogenesis via Gli3 proteolytic processing – Besse, Schneider-Manunoury
• Ciliary transition zone activation of phosphorylated Tctex-1 controls ciliary resorption, S- phase entry and fate of neural progenitors – Li, Sung
• A role for Tctex-1 in controlling primary cilium length – Palmer, Stephens
• Nde1 – mediated inhibition of ciliogenesis affects cell cycle re-entry – Kim, Tsiokas
• Cell shape and contractility regulate ciliogenesis in cell cycle-arrested cells – Pitaval, Thery

LiCl

• Lithium treatment elongates primary cilia in mouse brain and in cultured cells – Miyoshi, Asanuma
• Minerals and Osteoporosis – Rico
• Bipolar drugs may raise risk of osteoporosis – Goldman
• Lithium’s effect on bone mineral density – Zamani, Nasab

Microtubules

• Thermal Fractionation of Outer fiber doublet microtubules into A- and B-subfiber components: A- and B – Tubulin – Stephens
• Analysis of microtubule rigidity using hydrodynamic flow and thermal fluctuations – Venier, Pantaloni
• Taxol induced flexibility of microtubules and its reversal by MAP-2 Tau – Dye, Williams Jr
• Prediction of elastic properties of sperm flagella – Schoutens
• Structural insights into microtubule doublet interactions in axonemes – Downing, Sui
• Cyclical interactions between two outer doublet microtubules in split flagellar axonemes – Aoyama, Kamiya
• Flexural rigidity of singlet microtubules estimated from statistical analysis of their contour lengths and end to end distances – Mizushima –Sugano, Miki-Noumura
• Motility of triton-demembranated sea urchin sperm flagella during digestion by trypsin – Brokaw, Simonick
• Microtubule curvatures under perpendicular electric forces reveal a low persistence length – Huevel, Dekker
• Flexural rigidity of microtubules and actin filaments measured from thermal fluctuations in shape – Gittes, Howard
• Polyglutamylation and the fleer gene – Pathak, Drummond
• Dynamic of an anchored elastic filament in stokes flow
• Mechanochemical model of microtubule structure and self-assembly kinetics – VanBuren, Odde
• Rigidity of microtubules is increased by stabilizing agents – Mickey, Howard
• Flexural rigidity of individual microtu ules measured by a buckling force with optical traps – Kikumoto, Tashiro

AC6/cAMP

• Primary cilium-dependent mechanosensing is mediated by adenylyl cyclase 6 and cyclic AMP in bone cells
• The role of membrane microdomains in shaping B2-adrenergic receptor- mediated cAMP Dynamics – DiPilato, Zhang
• Dynamic Visualization of Signaling activities in Living Cells – Allen, Zhang
• The type 3 adenylyl cyclase is required for novel object learning and extinction of contextual memory: role of cAMP signaling in primary cilia – Wang, Storm

Basal body

• De novo formation of basal bodies during cellular differentiation of Naegleria gruberi: progress and hypotheses – Lee
• Centriole/basal body morphogenesis and migration during ciliogenesis in animal cells – Dawe, Gull
• Regulating the transition from centriole to basal body – Kobayashi, Dynlacht
• New Tetrahymena basal body protein components identify basal body domain structure – Kilburn, Winey
• The PCM- basal body/primary cilium coalition – Moser, Rattner

Matl’ info

• Focalcheck fluorescent Microsphere standards
• Tubulin tracker green reagent for live cell tubulin labeling (Oregon green 488 taxol, bis-acetate)

Cilia Length

• Regulation of cilia assembly, disassembly, and length by protein phosphorylation – Cao, Pan
• Primary Cilia and the Cell Cycle – Plotnikova, Golemis
• Primary cilia cycle in PtK1 cells: effects of colcemid and taxol on cilia formation and resorption – Jensen, Rieder
• Mechanisms regulating cilia growth and cilia function in endothelial cells – Abdul-Majeed, Nauli
• Identification of signaling pathways regulating primary cilium length and flow-mediated adaptation – Besschetnova, Shah
• Intraflagellar transport particle size scales inversely with flagellar length: revisiting the balance-point length control model – Engel, Marshall

WNT/HH

• Gli2a protein localization reveals a role for Iguana/DZIp1 in primary ciliogenesis and dependence of Hedgehog signal transduction on primary cilia in the zebrafish – kim, Ingham
• Intraflagellar transport protein 122 antagonizes sonic hedgehog signaling and controls ciliary localization of pathway components – Quin, Eggenschwiler
• Regulation of bone mass by Wnt signaling – Krishnan, MacDougald
• The primary cilium as hedgehog signal transduction machine – Goetz, Anderson
• WNT signaling in osteoblasts regulates expression of the receptor activator of NFkB ligand and inhibits osteoclastogenesis in vitro – Spencer, Genever
• Activation of the Wnt signaling pathway: a molecular mechanism for lithium action – Hedgepeth, Klein
• Subcellular spatial regulation of canonical Wnt signaling at the primary cilium – Lancaster, Gleeson
• LRP5- independent activation of Wnt signaling by lithium chloride increases bone formation and bone mass in mice – Clement-Lacrox, Rawadi

P.Cilia Flow

• Cilia driven fluid flow as an epigenetic cue for otolith biomineralization on sensory hair cells of the inner ear – Yu, Roy
• Polycystins 1 and 2 mediate mechanosensation in the primary cilium of kidney cells – Nauli, Zhou
• Primary cilia sensitize endothelial cells for fluid shear stress – Hierck, Poelman
• Time dependent deformations in bone cells exposed to fluid flow in vitro: investigating the role of cellular deformation in fluid flow induced signaling – Kwon, Jacobs
• Effect of flow and stretch on the [Ca] response of principal and intercalated cells in cortical collecting duct – Liu Satlin
• Primary cilia mediate mechanosensing in bone cells by a calcium-independent mechanism – Malone, Jacobs
• Microfluidic devices for studies of primary cilium mediated cellular response to dynamic flow conditions – Rydholm, Brismar
• Removal of the MDCK cell primary cilium abolishes flow sensing – Praetorium, Spring

Model/Mech

• Mechanosensitivity of bone cells to oscillating fluid flow induced shear stress may be modulated by chemotransport – Donahue, Jacobs
• A microstructurally informed model for the mechanical response of three-dimensional actin networks – Kwon, Jacobs
• Bending moments and elastic constants in cilia - Rikmenspoel, Sleigh
• Large deflections of a cantilever beam subjected to a cantilever beam subjected to a rotational distributed loading – Rao, Rao
• Large deflections of point loaded cantilevers with nonlinear behavior – Monasa
• Large deflexions of cantilevers by the electronic analogue computer – Khwaja
• Large and small deflections of a cantilever beam – Belendez, Belendez
• On the large deflection of Cantilever beams with end rotational load – Rao, Rao
• Large deflection of cantilever beams Bisshopp, Drucker
• Large deflections of cantilever beams of nonlinear materials – Lewis, Monasa
• Large deflections of cantilever beams of nonlinear elastic material under a combined loading – Lee
• On the curvature of an Euler-Bernoulli beam – Kopmaz, Gundogdu
• A Hydrodynamic mechanosensory hypothesis for brush border microvilli – Guo, Weinbaum
• Solving boundary value problems for ordinary differential equations in Matlab with BVP4c – Shampine, Reichelt
• Mechanical properties of primary cilia regulate the response to fluid flow – Rydholm, Brismar
• A finite element method for mechanical response for Hair cell ciliary bundles – Cotton, Grant
• Analysis and modeling of the primary cilium bending response to fluid shear – Schwartz, Bowser
• Mechanosensory role of primary cilia in vascular hypertension – Nauli, Hierck