Jacobs:Protocol Oil Red O Staining

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(New page: ==Overview== Protocol for Oil Red O Staining. Useful for identifying adipocytes. ==Materials== *Citrate concentrate solution *Oil Red O *99% isopropanol *Distilled water *Acetone *PBS *...)
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Revision as of 18:56, 24 April 2008

Contents

Overview

Protocol for Oil Red O Staining. Useful for identifying adipocytes.

Materials

  • Citrate concentrate solution
  • Oil Red O
  • 99% isopropanol
  • Distilled water
  • Acetone
  • PBS
  • 60% isopropanol
  • Hematoxylin
  • Whitman paper
  • Transfer pipets
  • Serological pipets/Pipet aid
  • Water bath
  • Timer
  • Waste beaker
  • 70% EtOH
  • Markers
  • Gloves

Solutions

  1. Citrate Working Solution (store in 4C for long term use)
    • 2mL citrate concentrate solution
    • 8mL deionized water
  2. Oil Red O Stock Solution
    • 300mg Oil Red O
    • 100mL 99% isopropanol
  3. Staining Solution
    • 6 parts of stock Oil Red O
    • 4 parts of distilled water
    • Allow to stand for 10min
    • Filter this solution with Whitman paper
    • Working solution is stable for 1-2 hr

Procedure

Notes

Used in Stanford for Tissue Engineering Lab Course (ME385B)and 2007 Winter TC workshop.

References

Contact

  • History: CRJ-EJC, last updated 2/21/07


or instead, discuss this protocol.

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