Jacobs:Protocol Oil Red O Staining

Overview

Protocol for Oil Red O Staining. Useful for identifying adipocytes.

Materials

• Citrate concentrate solution
• Oil Red O
• 99% isopropanol
• Distilled water
• Acetone
• PBS
• 60% isopropanol
• Hematoxylin
• Whitman paper
• Transfer pipets
• Serological pipets/Pipet aid
• Water bath
• Timer
• Waste beaker
• 70% EtOH
• Markers
• Gloves

Solutions

1. Citrate Working Solution (store in 4C for long term use)
   • 2mL citrate concentrate solution
   • 8mL deionized water
2. Oil Red O Stock Solution
   • 300mg Oil Red O
   • 100mL 99% isopropanol
3. Staining Solution
   • 6 parts of stock Oil Red O
   • 4 parts of distilled water
   • Allow to stand for 10min
   • Filter this solution with Whitman paper
   • Working solution is stable for 1-2 hr

Notes

Used in Stanford for Tissue Engineering Lab Course (ME385B)and 2007 Winter TC workshop.

Contact

• History: CRJ-EJC, last updated 2/21/07

discuss this protocol.